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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Beta2-microglobulin is a small (99 amino acids) protein present as the light chain of the major immunocompatibility complex receptors and also the light chain of the CD1 receptor complex. The protein plays a critical role in both receptor maturation and in antigen expression at the surface of immune cells. It is essential for HLA recognition and binding of peptides. Beta2-microglobulin is also involved as a partner of the human hemochromatosis protein and as such helps regulate iron levels.
Elevated levels of the protein in serum are a marker of leukemia and multiple myeloma. Elevated levels in urine indicate kidney damage, while elevated levels in CSF indicate meningitis or brain tumors.
|Assay Target||Beta-2 microglobulin|
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.