PerkinElmer
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Beef troponin AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for beef troponin enables the quantitative determination of beef troponin in food samples using a homogeneous AlphaLISA assay (no wash steps). This assay kit recognizes beef, bison, lamb, and pork meat but shows low cross reacitivity with chicken meat.

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Part Number Unit Size List Price Your Price Quantity
AL405HV 100 Assay Points 450.00 USD
AL405C 500 Assay Points 1391.00 USD
AL405F 5,000 Assay Points 9200.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Adulteration of food is the process in which food impurities are added to our foods. This process is highly regulated by departments of the US Government and other Governments around the world. One area of concern for many, usually from a health, ethical or cultural standpoint, is the contamination of vegan and vegetarian foods with animal meats. Another area of concern is the presence of meat in vegetarian feed for livestock or poultry. In a recent study, 10% of vegetarian products tested contained traces of animal meats. The AlphaLISA kit present here quantifies the amount of Troponin I, a skeletal muscle protein that is highly conserved within mammalian species, in meat (raw or cooked) potentially present in food and feed products.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Beef troponin
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Food Testing
Unit Size 100 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Manual AlphaLISA Beef Troponin Detection kit AL405 PDF  557 KB
Technical Note Detection and Quantification of Food Contaminants Using AlphaLISA PDF  1 MB
Event Society of Laboratory Automation & Screening (SLAS)