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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Beta-secretase 1 (BACE-1) is an aspartic protease encoded by the BACE-1 gene that is expressed ubiquitously throughout the body. BACE-1 has been identified as a necessary enzyme that functions with gamma-secretase to process of the amyloid precursor protein into the amyloid beta peptide, where the BACE-1 enzyme is responsible for the generation of the variable sizes of the peptide, particularly amyloid beta 1-40 and 1-42. As such, BACE1 is thought to be a valuable biomarker for Alzheimer’s disease, especially in the CSF. This AlphaLISA kit allows for the detection and quantification of BACE-1 in human serum, CSF, cell culture media and cell lysates.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 Assay Points|
This manual explains how to run the AlphaLISA no-wash human BACE-1 detection assay.