The AlphaLISA® immunoassay kit for human V-domain Ig suppressor of T cell activation (VISTA) enables the quantitative determination of human VISTA (B7-H5, PD-1H) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL3059HV||100 Assay Points||750.00 USD|
|AL3059C||500 Assay Points||1800.00 USD|
|AL3059F||5,000 Assay Points||14400.00 USD|
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
V-domain Ig suppressor of T cell activation (VISTA), also known as B7-H5 and PD-1H, is a transmembrane protein that functions as an immune checkpoint. VISTA is primarily express in white blood cells and can act either as a ligand or receptor on T-cells. Its main function is to inhibit T cell effector function and maintain peripheral tolerance. VISTA is upregulated in tumor-infiltrating lymphocytes and blocking it with an antibody has proven to stunt tumor growth in mouse melanoma models. HIV-infected individuals also display increased VISTA levels in monocytes.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 Assay Points|
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|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 128 KB|
|Application Note||Development of an AlphaLISA Assay for Screening Inhibitors of the TIM-3/Gal-9 Interaction||PDF 3 MB|
|Application Note||Protocol Optimization for Detection and Quantification of Membrane-Bound Proteins Using AlphaLISA Te||PDF 2 MB|
|Application Note||Quantifying Changes in CD28 and CTLA-4 Levels in Peripheral Blood Mononuclear Cells with AlphaLISA||PDF 1 MB|
|Application Note||Utilizing AlphaLISA Technology to Screen for Inhibitors of the CTLA-4 Immune Checkpoint||PDF 1 MB|
|Manual||AlphaLISA human VISTA Detection Kit||PDF 519 KB|
|Technical Note||AlphaLISA Assays for Immune Checkpoint Detection and Quantitation||PDF 532 KB|