The AlphaLISA® immunoassay kit for human AST enables the quantitative determination of human aspartate transaminase (AST) in buffered solution, cell culture media, and serum using a homogeneous AlphaLISA assay (no wash steps).
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Aspartate transaminase (AST) or aspartate aminotransferase, also known as AspAT/ASAT/AAT or serum glutamic oxaloacetic transaminase (SGOT), is an important enzyme in amino acid metabolism. AST catalyzes the reversible transfer of an α-amino group between aspartate and glutamate. AST is found in metabolically active organgs and issues, such as the liver, heart, skeletal muscle, kidneys, brain, and red blood cells. Clinically, serum levels of AST and ALT (alanine transaminase) and their ratio (AST/ALT ratio) are used as biomarkers to monitor liver health. This kit has been designed for the detection of human AST in serum and cell culture media.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 Assay Points|