The AlphaLISA® 4-1BB/4-1BBL binding kit is designed for the detection of binding activity between human 4-1BB and 4-1BBL, using a fast and simple homogeneous AlphaLISA assay (no wash steps). This assay can be used to screen for small molecules that inhibit binding, as a competitive ligand binding (CLB) assay to screen therapeutic blocking antibodies, and for potency assays.
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The AlphaLISA® human 4-1BB and human 4-1BBL binding assay uses anti-6xHis AlphaLISA Acceptor beads to capture the His-tagged 4-1BB and Streptavidin-coated donor beads to capture the biotinylated 4-1BBL. Donor beads and Acceptor beads come into proximity through 4-1BB binding to 4-1BBL. Excitation of the Donor beads provokes the release of singlet oxygen that triggers a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
4-1BB is a member of the TNF superfamily. It is a type 2 transmembrane glycoprotein receptor with approximate molecular weight of 30 kDa and expressed mostly on activated T Lymphocytes. The expression is also reported on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. It has many different names such as CD137 and TNFRSF9. 4-1BB binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells. This interaction co‑stimulates the proliferation, activation, and/or survival of the 4-1BB expressing cells. 4-1BB activation enhances CD8+ T cell and NK cell mediated anti-tumor immunity. Soluble forms of 4-1BB and 4-1BB Ligand circulate at elevated levels in the serum of rheumatoid arthritis and hematologic cancer patients, respectively.
|Assay Target||4-1BBL, 4-1BB|
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|