The AlphaScreen® SureFire® total Topoisomerase II-α assay is a sandwich immunoassay for quantitative detection of Topoisomerase II-α (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
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Alpha SureFire® kits can be used for:
Formats:
In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.
| Assay Target | Topo IIα |
|---|---|
| Assay Target Class | Protein |
| Automation Compatible | Yes |
| Detection Method | Alpha |
| Experimental Type | In vitro |
| Product Brand Name | AlphaScreen SureFire |
| Quantity in a Package Amount | 10000.0 Units |
| Shipping Condition | Blue Ice |
| Target Species | Human |
| Unit Size | 10,000 assay points |
