The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser235/236) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser235/236) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately, in addition to the AlphaScreen SureFire kit.
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Alpha SureFire® kits can be used for:
Formats:
In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
| Assay Pathway | PI3K/Akt/mTOR |
|---|---|
| Assay Target | S6RP |
| Assay Target Class | Phosphoprotein |
| Automation Compatible | Yes |
| Detection Method | Alpha |
| Experimental Type | In vitro |
| Molecular Modification | Phosphorylation |
| Product Brand Name | AlphaScreen SureFire |
| Quantity in a Package Amount | 10000.0 Units |
| Second Messenger Release | Protein phosphorylation |
| Shipping Condition | Blue Ice |
| Unit Size | 10,000 assay points |
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
