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The AlphaLISA® SureFire® Ultra™ p-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGF Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA® SureFire® Ultra™ kits are compatible with:
Alpha SureFire® kits can be used for:
|Assay Pathway||Receptor signaling|
|Assay Target||VEGF2 Receptor|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA SureFire Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||100 assay points|