Extra control lysate for the AlphaLISA SureFire Ultra total VEGFR-2 assay. This control is already provided in the AlphaLISA SureFire Ultra total VEGF R2 assay kit, but can also be ordered separately.
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For research use only. Not for use in diagnostic procedures.
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This control lysate is intended for use with the AlphaLISA SureFire Ultra total VEGFR-2 assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HUVEC cells were grown to confluence in T175 flasks, and then serum-starved overnight. The following day the media was removed, and the cells were treated with 100 ng/mL VEGF for 10 minutes. The agonist was removed, and the cells were lysed with 4 mL of freshly prepared 1X Lysis buffer, with shaking for 10 minutes.
|Assay Target||VEGF2 Receptor|
|Assay Target Class||Protein|
|Shipping Condition||Blue Ice|