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AlphaLISA SureFire Ultra Total STAT3 Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ Total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

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Part Number List Price Your Price Quantity
ALSU-TST3-A500 1605.00 USD
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Overview

The AlphaLISA® SureFire® Ultra™ Total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA® SureFire® Ultra™ kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies


Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Pathway JAK/STAT
Assay Target STAT3
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra
Shipping Condition Blue Ice
Target Species Human
Unit Size 500 Assay Points

Resources, Events & More

All (6)
Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Application Note Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra PDF  2 MB
Manual Protocol for AlphaLISA SureFire Ultra Total STAT3 Assay Kits PDF  781 KB
Poster Rapid homogeneous detection systems for cellular signaling pathways PDF  805 KB
Flyer Alpha SureFire Technology PDF  720 KB
Event Society of Laboratory Automation & Screening (SLAS)