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AlphaLISA SureFire Ultra STAT1 pS727 HV, 100 Assay Points

The AlphaLISA® SureFire® Ultra p-STAT1 (Ser727) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Ser727) in cellular lysates using Alpha Technology.

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Part Number Unit Size List Price Your Price Quantity
ALSU-PST1-B-HV 100 Assay Points 475.00 USD
ALSU-PST1-B500 500 Assay Points 1605.00 USD
ALSU-PST1-B10K 10,000 Assay Points 8900.00 USD
ALSU-PST1-B50K 50,000 Assay Points 27600.00 USD
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Overview

The AlphaLISA® SureFire® Ultra p-STAT1 (Ser727) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Ser727) in cellular lysates using Alpha Technology.

This kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.

In the AlphaLISA® SureFire® Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA® SureFire® Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Pathway JAK/STAT
Assay Target STAT1
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Shipping Condition Blue Ice
Unit Size 100 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Application Note Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra PDF  2 MB
Manual Protocol for AlphaLISA® SureFire® Ultra STAT1 (p-Ser727) HV Assay Kits PDF  554 KB
Poster Rapid homogeneous detection systems for cellular signaling pathways PDF  805 KB
Flyer Alpha SureFire Technology PDF  720 KB