The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.
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The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology.
In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA® SureFire® Ultra™ kits are compatible with:
Alpha SureFire® kits can be used for:
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Phosphoprotein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA SureFire Ultra|
|Second Messenger Release||Protein phosphorylation|
|Shipping Condition||Blue Ice|
|Unit Size||50,000 Assay Points|
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|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 124 KB|
|Application Note||Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra||PDF 2 MB|
|Manual||Protocol for AlphaLISA SureFire Ultra p-JNK 1/2/3 Assay Kits||PDF 775 KB|
|Poster||Rapid homogeneous detection systems for cellular signaling pathways||PDF 805 KB|
|Flyer||Alpha SureFire Technology||PDF 720 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)|