PerkinElmer
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AlphaLISA SureFire Ultra Total GAPDH Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ Total GAPDH assay is a sandwich immunoassay for quantitative detection of GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for cell number.

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Part Number Unit Size List Price Your Price Quantity
ALSU-TGAPD-A-HV 100 Assay Points 475.00 USD
ALSU-TGAPD-A500 500 Assay Points 1605.00 USD
ALSU-TGAPD-A10K 10,000 Assay Points 8800.00 USD
ALSU-TGAPD-A50K 50,000 Assay Points 27500.00 USD
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Overview

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA® SureFire® Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

AlphaLISA® SureFire® Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Assay Target GAPDH
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA SureFire Ultra
Quantity in a Package Amount 100.0 Units
Shipping Condition Blue Ice
Target Species Human
Unit Size 100 Assay Points

Resources, Events & More

All (6)
Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Application Note Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra PDF  2 MB
Manual AlphaLISA SureFire GAPDH Detection Kit (HV - High Volume) PDF  516 KB
Poster Rapid homogeneous detection systems for cellular signaling pathways PDF  805 KB
Flyer Alpha SureFire Technology PDF  720 KB
Event Society of Laboratory Automation & Screening (SLAS)