The AlphaLISA® SureFire® Ultra™ p-AKT1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.
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Formats:
In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA SureFire Ultra kits are compatible with:
Alpha SureFire kits can be used for:
Assay Pathway | PI3K/Akt/mTOR |
---|---|
Assay Target | Akt1/2/3 |
Assay Target Class | Phosphoprotein |
Automation Compatible | Yes |
Detection Method | Alpha |
Product Brand Name | AlphaLISA SureFire Ultra |
Quantity in a Package Amount | 500.0 Units |
Shipping Condition | Blue Ice |
Target Species | Human |
Unit Size | 500 Assay Points |