Bead set containing streptavidin-coated Alpha Donor beads and unconjugated AlphaScreen® Acceptor beads for creating your own Alpha no-wash assay. Unconjugated beads are amine-reactive, and can be used to create beads conjugated to your own biomolecule-of-interest.
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Conjugation of biomolecules to AlphaScreen beads is a very simple procedure. Unconjugated beads are readily available to facilitate access to, and allow the creation of, custom assay formats not commercially available or of a proprietary nature. Note that 1 mg translates into 2,000 assay points based on a 25 µL reaction volume and final bead concentration of 20 µg/mL.
AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym ""Alpha"" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Product Brand Name||AlphaScreen|
|Shipping Condition||Blue Ice|
|Unit Size||1 kit|
|Resource Type||File Name||File Format|
|Brochure||Alpha Technology Solutions||PDF 4 MB|
|Brochure||Alpha product listing||PDF 124 KB|
|Brochure||Handle Large Biomolecular Interactions With Ease||PDF 798 KB|
|Guide||Alpha Protein-Protein Interaction Quick Start Guide||PDF 380 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)|