The aequorin plasmid, which expresses the aequorin protein under the control of the CMV promoter, has been developed to convert mammalian cell lines into AequoScreen compatible cell lines.
Item(s) added to cart.
Restriction for product sale could exist in some countries.
Please enter valid quantity
Please log in to add favorites.
NULL OR EMPTY CART
The aequorin plasmid (+Ga16), which expresses the aequorin protein and the promiscuous G alpha16 protein under the control of the CMV promoter, has been developed to convert mammalian cell lines into AequoScreen® compatible cell lines. It is a powerful tool for the rapid development and analysis of cell lines, already expressing a recombinant or endogenous G-Protein coupled receptor of choice, to a luminescence readout. The aequorin plasmid (+Ga16) particularly addresses GPCRs of the Gs and Gi family enabling them to modulate intracellular calcium concentration. Terms and conditions apply.
|Assay Target Type||Cell line|
|Assay Validated||Calcium Luminescence|
|G-Alpha Coupling Protein||Ga16|
|Product Brand Name||AequoScreen|
|Second Messenger Release||Calcium flux|
|Shipping Condition||Dry Ice|
|Unit Size||10 µg|
Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which control its activity.
Aequorin and Photina cell lines – the alternative calcium flux assay. PDL-coated microplates improve your performance
We have shown here that signal intensity for the selected catalog aequorin cell lines is strong enough to allow its measurement by the non-luminescence dedicated FLIPR