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The aequorin plasmid, which expresses the aequorin protein under the control of the CMV promoter, has been developed to convert mammalian cell lines into AequoScreen® compatible cell lines. It is a powerful tool which enables the rapid development and analysis of cell lines, already expressing a recombinant or endogenous G-protein coupled receptor of choice, to a luminescence readout. The aequorin plasmid targets cells expressing any protein that modulates intracellular calcium concentration (e.g., GPCRs within the Gq family or GPCRs that couple via a promiscuous G-Protein / chimera).
|Assay Target Type||Cell line|
|Assay Validated||Calcium Luminescence|
|Product Brand Name||AequoScreen|
|Second Messenger Release||Calcium flux|
|Shipping Condition||Dry Ice|
|Unit Size||10 µg|
Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which control its activity.
Aequorin and Photina cell lines – the alternative calcium flux assay. PDL-coated microplates improve your performance
We have shown here that signal intensity for the selected catalog aequorin cell lines is strong enough to allow its measurement by the non-luminescence dedicated FLIPR