PerkinElmer
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Aβ(1-40) (human) High Specificity LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra Human Aβ(1-40) High Specificity Detection Kit is designed for detection and quantitation of human Aβ(1-40) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

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TRF1345C
500 Assay Points
796.00 USD
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10,000 Assay Points
10600.00 USD
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Detail Information

The LANCE® Ultra Human Aβ(1-40) High Specificity Detection Kit is designed for detection and quantitation of human Aβ(1-40) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Amyloid beta (Aβ) is a short peptide derived from a transmembrane protein, the amyloid precursor protein (APP), by proteolysis. The β- and γ-secretases cleave the respective N- and C-terminal ends of the Aβ sequence, liberating the Aβ peptide from APP. Aβ40 is the major species of Aβ produced by neurons and other cells, and accounts for over 70% of total Aβ produced, while the remainder is typically composed Aβ42 and other size variants ranging from 36 to 43 AA in length. Interestingly, it has been observed by some that the Aβ40/Aβ42 ratio increased in cerebrospinal fluid of AD patients. Aβ40 could be an important biomarker for detecting Alzheimer’s disease progression. This AlphaLISA kit has been designed to detect Aβ40 in cell culture supernatants and cerebrospinal fluid.

Specifications

Assay Target Aβ1-40
Assay Target Class Peptide
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Central Nervous System
Unit Size 500 Assay Points