4CN Plus substrate detects HRP and is ideal for western blotting applications. It produces dark purple precipitate in the prescence of HRP and is 10 times more sensitive than standard 4CN(4-chloro-1-napthol) formulations.
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4CN Plus is a chromogenic substrate that can be used for both nucleic acid detection as well as western blotting. The amount of reagents supplied is enough to provide 750 mL of working substrate solution which is enough reagent for 3000 cm2 of membrane. 4CN Plus has been formulated for use on Polyscreen™ PVDF Transfer Membrane, nitrocellulose membranes and the nylon membranes Gene Screen™ Hybridization Transfer Membrane and Gene Screen Plus® Hybridization Transfer Membrane.
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4CNPlus is a modified, highly sensitive 4CN (4-chloro-1-naphthol) formulation designed for the chromogenic detection of horseradish peroxidase (HRP). The sensitivity of 4CN Plus is approximately 10X that of conventional formulations of 4CN and is equal or slightly better than diaminobenzidine (DAB).