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Technical Note

AlphaLISA in Urine: Detecting Soluble TNFR1 in a Complex Sample Matrix

Introduction

Biomarkers provide insight into the presence and progression of disease states. Tumor necrosis factor 1 (TNF1) is a transmembrane protein that can be released by extracellular proteolytic cleavage to function as cytokines. Increased levels of soluble TNFR1 is an indicator for disease states such as infection, type 2 diabetes, and can be used as a predictive marker for progression to advanced chronic kidney disease or end-stage renal disease.

Typically, wash-based ELISAs are used to quantify increased levels of soluble TNFR1 from complex patient samples. While sensitive, ELISAs often require large amounts of samples and can be very labor intensive. AlphaLISA® is a homogenous bead-based assay. This technical note demonstrates how to quantify levels of TNFR1 in human urine samples by comparing normal patient donors to type 2 diabetics.

See how AlphaLISA technology provides a rapid, homogeneous, bead-based assay format for the quantification of an analyte in complex matrices.