PerkinElmer

TR-FRET Toolbox Reagents

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Design your own assay by choosing your preferred donor- and acceptor-labeled reagents. The versatility of LANCE® TR-FRET technology allows the assembly of donor and acceptor dye pairs for use in protein binding and other assays. This is assay design at its most flexible -- featuring the most common affinity biomolecules and europium chelate-labeling reagents.

LANCE toolbox reagents can be used to design a variety of assays, including kinase, protein-protein interaction, protein-nucleic acid interaction, protein-small molecule interaction, epigenetic, receptor-ligand binding, nuclear receptor, receptor dimerization, ubiquitination, and other post-translational modifications, protease, and other binding and enzymatic assays.

Products & Services (88)
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Application
  • Enzymatic (6)
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  • Time-Resolved Fluorescence (TRF) (81)
  • TR-FRET (81)
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  • Eu-W1024 (49)
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Automation Compatible
  • Yes (88)
Experimental Type
  • In vitro (87)
Format
  • Microplates (87)

1-12 of 88 Products & Services

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  • LANCE® Eu-W1024 phospho-(Ser) 14-3-3 binding motif antibody, 10 µg

    Europium-labeled anti-phospho-(Ser) 14-3-3 motif antibody, for use in LANCE® TR-FRET kinase assays.
    List Price : 3544.00 USD
  • LANCE Ultra ULight™-JAK-1 (Tyr1023) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from amino acids 1015-1027 of human Janus kinase 1 (JAK-1). The ULight-JAK-1 peptide can be phosphorylated at Tyr1023 by JAK3 and several other tyrosine kinases. Peptide sequence: CAGAGAIETDKEYYTVKD
    Price Range : 408.00 USD - 1250.00 USD
  • LANCE Ultra Europium-anti-phospho-p70 S6K (Thr389) Antibody

    Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human p70 S6K phosphorylated at Thr389. p70 S6K is phosphorylated in vivo by mTOR. p70 S6K peptides are also phosphorylated by several kinases including COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1 and TAOK2. Sequence motif recognized: LGFTYVAP
    Price Range : 1597.00 USD - 7400.00 USD
  • LANCE Ultra Europium-anti-phospho-Histone H3 (Ser10) Antibody

    Mouse monoclonal antibody labeled with the LANCE Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated by Ser10. Histone H3 peptides can be phosphorylated by several kinases, including Aurora B. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG
    Price Range : 2130.00 USD - 7400.00 USD
  • LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide Antibody

    Mouse monoclonal antibody labeled with the LANCE® Europium chelate. This antibody detects phospho-Ser in a synthetic peptide (RFARKGSLRQKNV) only after the peptide is phosphorylated by PKC or PKA kinases. The synthetic peptide is derived from amino acids 19-31 of human PKCα in which Ala25 is mutated to Ser. Sequence motif recognized: RFARKG-pS-LRQKNV
    Price Range : 2130.00 USD - 7400.00 USD
  • ULight anti-mouse IgG

    ULight™ labeled anti-mouse IgG antibody for LANCE® TR-FRET assays. The anti-mouse IgG antibody is a purified goat polyclonal antibody.
    Price Range : 446.00 USD - 11300.00 USD
  • LANCE Ultra ULight™-eIF4E-binding protein 1 (Thr37/46) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Synthetic peptide containing residues surrounding Thr37 and Thr46 of human eukaryotic translation initiation factor 4E-binding protein 1; phosphorylation sites: Thr37 and Thr46. The ULight-eIF4E-binding protein 1 peptide can be phosphorylated at Thr37/46 by mTOR and many kinases. Phosphorylation motif: STTPGGTLFSTTPG
    Price Range : 417.00 USD - 899.00 USD
  • LANCE Ultra Europium-anti-acetyl-Histone H3 Lysine 27 (H3K27ac) Antibody

    Europium-labeled monoclonal antibody recognizing human Histone H3 acetylated at lysine 27 (H3K27ac). For use in LANCE® Ultra epigenetic writer and eraser assays.
    Price Range : 3632.00 USD - 9100.00 USD
  • LANCE Ultra Europium-anti-phospho-MEK1/2 (Ser217/221) Antibody

    Rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human MEK1 phosphorylated at Ser217 and Ser221. It also reacts with MEK1 singly phosphorylated at Ser217 or Ser221. MEK1 is phosphorylated in vivo by RAF1 and COT. Homologous phosphorylation sites are found in MEK2. Sequence motif recognized: GQLIDSMANSFVG
    Price Range : 2275.00 USD - 7400.00 USD
  • LANCE Ultra ULight™-DNA Topoisomerase 2-alpha (Thr1342) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Synthetic peptide containing residues surrounding Thr1342 of human DNA Topoisomerase 2-alpha; phosphorylation site: Thr1342. The ULight-DNA Topoisomerase 2-alpha (Thr1342) peptide can be phosphorylated by PLK3, CK2α and other kinases. Phosphorylation motif: DEKTDDE
    Price Range : 409.00 USD - 899.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-W1024 Anti-c-Myc

    Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.
    Price Range : 310.00 USD - 14900.00 USD
  • ULight Anti-C-tag

    ULight™ labeled anti-C-tag antibodies for LANCE® TR-FRET assays. This mouse monoclonal IgG1 antibody recognizes N-terminal and C-terminal Protein C tagged fusion proteins. The anti-C-tag antibody can be used to capture C-tag proteins, and can be used in conjunction with LANCE Europium-labeled reagents to create LANCE TR-FRET assays.
    Price Range : 453.00 USD - 11100.00 USD
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