PerkinElmer

Protein Kinase Research

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Cancer, diabetes, and Alzheimer’s research can benefit from the study of phosphorylation and protein kinase activity. Because of their role in signal transduction, kinase activity detection and characterization is significant for drug discovery.

We offer a range of end-point and real-time kinetic protein kinase assays, technologies, and instruments that allow rapid kinase detection, screening, and mechanism-of-action studies.

Our offerings include:

  • Labeled substrates for kinetic assays and antibody-free screening
  • AlphaLISA® kits for highly-sensitive detection of cellular phosphorylation
  • LANCE® TR-FRET protein kinase assays for rapid assay selection
  • Fast and simple assays using pre-validated peptide and protein substrates derived from biologically-relevant targets
  • Multimode plate readers for maximal speed and throughput independent of technology platform

Regulation and inhibition of kinase activity can open new pathways for therapeutics, especially in conditions related to cell signaling and growth factors.

Whether you work with serine/threonine-specific, tyrosine-specific, histidine-specific, or mixed kinases, our kinase activity assays and technologies enable rapid assay selection and accelerate drug development.

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  • Yes (231)
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  • Microplates (134)
Maximum Temperature (Celsius)
  • 40-50 (2)
  • 50-60 (2)
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"Protein Kinase Research" 1-50 of 289 Products and Services

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EnSight Multimode Plate Reader

EnSight Multimode Plate Reader

The EnSight multimode plate reader combines labeled & image-based cytometry technologies in a single benchtop platform. Imaging & online data analysis is fast: a 384-well plate can be imaged in less than 5 mins. The system’s modular design, combined with workflow-based Kaleido data acquisition & analysis software, gives a truly versatile plate reader that gets users productive quickly.
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr421/Ser424) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr421/Ser424) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr421/Ser424) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit
Price Range : 1538.00 USD - 16600.00 USD
SureFire principle

AlphaScreen SureFire Akt 123 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1703.00 USD - 18200.00 USD
PerkinElmer

ProfilerPro Kinase Peptide Substrate 32, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 32 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 757.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-SMAD3 (Ser423/425) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-SMAD3 (Ser423/425) kits are designed for the detection of SMAD3 (phosphorylated at residue S423/S425) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1512.00 USD - 6800.00 USD

LANCE Ultra ULight™-poly GT

Poly Glu-Tyr (4:1) labeled with ULightTM dye. This random polymer is a Tyrosine kinase substrate.
Price Range : 330.00 USD - 900.00 USD
AlphaLISA SureFire Ultra kits

Phospho-Syk (Tyr352) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ phospho-Syk assay is a sandwich immunoassay for quantitative detection of Syk (phosphorylated on Tyr352) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 30400.00 USD

AlphaScreen Phosphotyrosine (PY20) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PY20 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 188.00 USD - 14400.00 USD
AlphaLISA SureFire Ultra kits

Phospho-ErbB2 (Tyr1221/1222) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ p-ErbB2 (Tyr1221/1222) assay is a sandwich immunoassay for quantitative detection of phospho-ErbB2 (phosphorylated on Tyr1221/1222) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 29200.00 USD
SureFire principle

AlphaScreen SureFire Phospho-beta catenin (Ser45) Assay Kit

The AlphaScreen® SureFire® p-β-catenin (Ser45) assay is a sandwich immunoassay for quantitative detection of phospho-β-catenin (phosphorylated on Ser45) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD
SureFire principle

AlphaScreen SureFire Akt 1 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD
SureFire principle

AlphaScreen SureFire IkBalpha (p-Ser32/Ser36) Assay Kit

The AlphaScreen® SureFire® p-IκBα (Ser32/36) assay is a sandwich immunoassay for quantitative detection of phospho-IκBα (phosphorylated on Ser32/36) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD
SureFire principle

AlphaScreen SureFire ELK1 (p-Ser383) Assay Kit

The AlphaScreen® SureFire® p-ELK1 (Ser383) assay is a sandwich immunoassay for quantitative detection of phospho-ELK1 (phosphorylated on Ser383) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD

Guanosine 5`-(γ-thio)triphosphate, [35S]-, 10mM Tricine (pH 7.6), 10mM DTT, 1.0mC/mL

Guanosine triphosphate, labeled on the gamma phosphate group with 35S (GTPγS).
Price Range : 1484.00 USD - 2606.00 USD
AlphaLISA SureFire Ultra kits

Total ZAP-70 AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ total ZAP-70 assay is a sandwich immunoassay for quantitative detection of total ZAP-70 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay in detection of phospho-ZAP-70.
Price Range : 504.00 USD - 30400.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-CREB (Ser133) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 29200.00 USD
PerkinElmer

LANCE® Eu-W1024 Anti-phosphotyrosine (PT66)

Europium-labeled anti-phosphotyrosine (PT66) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 1087.00 USD - 9500.00 USD
SureFire principle

AlphaScreen SureFire Akt 1/2 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt 1/2 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt 1/2 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD
SureFire principle

AlphaScreen SureFire InsulinR (p-Tyr1150;Tyr1151) Assay Kit

The AlphaScreen® SureFire® p-Insulin Receptor (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-Insulin Receptor (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1703.00 USD - 18200.00 USD

LANCE Ultra ULight™-PKC substrate

PKC substrate (sequence: CRFARKGSLRQKNV) labeled with ULight™ dye. Synthetic peptide derived from amino acids 19-31 of PKC?.
Price Range : 417.00 USD - 1135.00 USD

LANCE Ultra Europium-anti-phospho-Myelin Basic Protein (MBP)

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Thr232 in myelin basic protein (MBP; Swiss-Prot: P02686). MBP can be phosphorylated by a variety of kinases including Erk1/Erk2. Sequence motif recognized: FFKNIVTPRpTPPPSQGK
Price Range : 306.00 USD - 6300.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-Insulin Receptor β (Tyr1150/1151) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-Insulin Receptor β (Tyr1150/1151) in a multiplexed assay.
Price Range : 378.00 USD - 22800.00 USD
PerkinElmer

ProfilerPro Kinase Peptide Substrate 27, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 27 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 757.00 USD
SureFire principle

AlphaScreen SureFire CHK2 (p-Thr68) Assay Kit 

The AlphaScreen® SureFire® p-CHK2 (Thr68) assay is a sandwich immunoassay for quantitative detection of phospho-CHK2 (phosphorylated on Thr68 in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD
SureFire principle

AlphaScreen SureFire ALK (p-Tyr1604) Assay Kit

The AlphaScreen® SureFire® p-ALK (Tyr1604) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1604) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD
SureFire principle

AlphaScreen SureFire EGFR (p-Tyr1068) Assay Kit

The AlphaScreen® SureFire® p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGFR (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1703.00 USD - 18200.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-eIF4E (Ser209) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-eIF4E (Ser209) in a multiplexed assay.
Price Range : 378.00 USD - 22800.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 29200.00 USD
SureFire principle

AlphaScreen SureFire Phospho-MEK 1 Kit

The AlphaScreen® SureFire® p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1703.00 USD - 18200.00 USD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

DELFIA Eu-N1 Anti-Phosphotyrosine (PY20)

Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in DELFIA time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.
Price Range : 339.00 USD - 3387.00 USD
AlphaLISA SureFire Ultra kits

Phospho-JNK1/2/3 (Thr183/Tyr185) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.
Price Range : 504.00 USD - 29200.00 USD
AlphaLISA SureFire Ultra kits

Phospho-p38 MAPK (Thr180/Tyr182) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Tyr180/182) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 29200.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total NFκB LANCE Ultra TR-FRET Detection Kit

LANCE Ultra total NFκB kits are designed for the detection of NFκB (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-NFκB assay.
Price Range : 1512.00 USD - 6800.00 USD
SureFire principle

AlphaScreen SureFire p-Akt 1/2/3 (Thr308) research kit

The AlphaScreen® SureFire® p-Akt1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1703.00 USD - 18200.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT5 (Tyr694/699) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. This kit replaces ALSU-PST5-A-HV. ALSU-PST5-A-HV is no longer available as the antibody(ies) have been discontinued by the supplier. A new antibody pair has been selected and validated to make this new version, with a similar or slightly improved performance over the initial version. Comparative data are included in the manuals of the new kits.
Price Range : 504.00 USD - 29200.00 USD
SureFire principle

AlphaScreen SureFire cJun (p-Ser63) Assay Kit

The AlphaScreen® SureFire® p-c-Jun (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-c-Jun (phosphorylated on Ser63) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit

The AlphaLISA® SureFire® Ultra p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 29200.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT6 (Tyr641) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT6 (Tyr641) assay is a sandwich immunoassay for quantitative detection of phospho-STAT6 (phosphorylated on Tyr641) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 29200.00 USD
SureFire principle

AlphaScreen SureFire CREB (p-Ser133) Assay Kit

The AlphaScreen® SureFire® p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1703.00 USD - 18200.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SYK (Tyr525/526) Assay Kit

The AlphaLISA® SureFire® Ultra p-SYK (Tyr525/526) assay is a sandwich immunoassay for quantitative detection of phospho-SYK (phosphorylated on Tyr525/526) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 29200.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-Ribosomal Protein S6 (Ser240/244) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-Ribosomal Protein S6 (Ser240/244) in a multiplexed assay.
Price Range : 378.00 USD - 22800.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total AKT1 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total AKT1 kits are designed for the detection of total AKT1 (phosphorylated and unphosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-AKT1, and is compatible with both adherent and suspension cells.
Price Range : 1512.00 USD - 6800.00 USD

LANCE Ultra ULight™-Histone H3 (Thr3/Ser10) Peptide

ULight™-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence : CSERRPHFPQFSYSASGTAR
Price Range : 306.00 USD - 1250.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-p38 MAPK (Thr180/Tyr182) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-p38 MAPK (Thr180/Tyr182) in a multiplexed assay.
Price Range : 378.00 USD - 22800.00 USD
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser112) Kit

The AlphaScreen® SureFire® p-BAD (Ser112) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser112) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD
AlphaLISA SureFire Ultra kits

Phospho-IGF Receptor β (Tyr1135/1136) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra p-IGF Receptor β (Tyr1135/1136) assay is a sandwich immunoassay for quantitative detection of phospho-IGF Receptor β (phosphorylated on Tyr1135/1136) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 29200.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-AKT1/2/3 (Thr308) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 29200.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT3 (Tyr705) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT3 (Tyr705) in a multiplexed assay.
Price Range : 378.00 USD - 22800.00 USD
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr389) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1703.00 USD - 18200.00 USD

LANCE Ultra ULight™-poly GAT

Poly Glu-Ala-Tyr (1:1:1) labeled with ULight™ dye. This random polymer is a Tyrosine kinase substrate.
Price Range : 436.00 USD - 999.00 USD

"Protein Kinase Research" 1-50 of 289 Products and Services

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