PerkinElmer

LANCE TR-FRET

OF

LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

Assays for Biomarker Detection
Assays for Biomarker Detection

Assays for Biomarker Detection

Biomarker detection is key for advancing precision medicine. An array of biomarker detection kits are available utilizing our LANCE technologies in therapeutic areas including:
  • Angiogenesis
  • Animal Health
  • Autophagy
  • Biologics/Bioprocess
  • Cancer
  • Cardiovascular
  • Central nervous system
  • Inflammation
  • Metabolic
OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. http://www.perkinelmer.com/LANCE/ASK.

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1-12 of 363 Products & Services

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  • LANCE Detection Kit

    IGF-1 (rat) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Rat IGF-1 Detection Kit is designed for detection and quantitation of rat insulin-like growth factor 1 (IGF-1) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 806.00 USD - 10700.00 USD
  • LANCE Detection Kit

    IL-31 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-31 Detection Kit is designed for detection and quantitation of human interleukin-31 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 806.00 USD - 10700.00 USD
  • IgA (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IgA Detection Kit is designed for detection and quantitation of human immunoglobulin A in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11100.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra NFκB Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-NFκB (Ser536) and Total NFκB detection assays.
    List Price : 365.00 USD
  • AlphaLisa Analyte 2

    IgM (human), lyophilized (LANCE)

    Human IgM standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human IgM TR-FRET Detection Kit, but can also be ordered separately.
    List Price : 243.00 USD
  • Protein

    IGF-1 (rat), lyophilized (LANCE)

    Rat IGF-1 (insulin-like growth factor 1) standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra rat IGF-1 Detection Kit, but can also be ordered separately.
    List Price : 308.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra STAT1 Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-STAT1 and total STAT1 detection assays.
    List Price : 365.00 USD
  • LANCE Detection Kit

    IL-4 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-4 Detection Kit is designed for detection and quantitation of human interleukin 4 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11100.00 USD
  • ULight Anti-C-tag

    ULight™ labeled anti-C-tag antibodies for LANCE® TR-FRET assays. This mouse monoclonal IgG1 antibody recognizes N-terminal and C-terminal Protein C tagged fusion proteins. The anti-C-tag antibody can be used to capture C-tag proteins, and can be used in conjunction with LANCE Europium-labeled reagents to create LANCE TR-FRET assays.
    Price Range : 453.00 USD - 11100.00 USD
  • LANCE standard

    IL-6 (human), lyophilized (LANCE)

    Human Interleukin 6 (IL-6) standard, lyophilized, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra human IL-6 Detection Kit, but can be ordered separately.
    List Price : 250.00 USD
  • Europium-labeled antibody for LANCE assays

    Eu-anti-V5 (LANCE TR-FRET)

    Europium-labeled anti-V5 antibody, for capturing V5-tagged proteins in LANCE® TR-FRET assays. This Europium-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® ULight or SureLight APC reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
    Price Range : 322.00 USD - 9100.00 USD
  • IgG (bovine) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Bovine IgG Detection Kit is designed for detection and quantitation of bovine IgG in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 1494.00 USD - 9900.00 USD
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