PerkinElmer

LANCE TR-FRET

OF

LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. http://www.perkinelmer.com/ASK/LANCE.

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"LANCE TR-FRET" 1-50 of 363 Products & Services

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LANCE Detection Kit

IL-12/p40 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-12/p40 Detection Kit is designed for detection and quantitation of human interleukin-12/p40 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 830.00 USD - 11100.00 USD

ULight-anti-V5 (LANCE TR-FRET)

Anti-V5 tag antibody labeled with ULight™ dye. This ULight-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® Europium-labeled reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
Price Range : 433.00 USD - 11000.00 USD
LANCE Detection Kit

Apo-CIII (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Apo-CIII Detection Kit is designed for detection and quantitation of ApoC3 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 806.00 USD - 10700.00 USD
Protein

PTEN (human), lyophilized (LANCE)

Human PTEN standard, lyophilized, for use in running standard curves in LANCE Ultra TR-FRET detection assays. This standard is already provided in the LANCE Ultra Human PTEN Detection Kit, but can be ordered separately.
List Price : 317.00 USD

ULight anti-FITC

ULight™ labeled anti-FITC antibody for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.
Price Range : 446.00 USD - 11300.00 USD

LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 9/Lysine 27 (H3K9/K27) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 that is unmodified at lysine 9 and lysine 27 (H3K9/K27). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3632.00 USD - 9100.00 USD

ANGPTL4 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human ANGPTL4 Detection Kit is designed for detection and quantitation of human angiopoietin-like 4 protein in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 830.00 USD - 11100.00 USD
LANCE Detection Kit

GAD67 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human GAD67 Detection Kit is designed for detection and quantitation of Glutamic acid decarboxylase 67 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 806.00 USD - 10700.00 USD

Leptin (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Leptin Detection Kit is designed for detection and quantitation of human leptin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 830.00 USD - 11000.00 USD
LANCE Detection Kit

E cadherin (human) LANCE Ultra TR-FRET Detection Kit

Human E-cadherin standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human E-cadherin TR-FRET Detection Kit, but can also be ordered separately.
Price Range : 806.00 USD - 10700.00 USD

ULight anti-c-myc antibody

ULight labeled anti-c-myc antibody for LANCE® TR-FRET assays.
Price Range : 446.00 USD - 11300.00 USD

LANCE Ultra Europium-anti-acetyl-Histone H4 Lysine (H4Kac pan) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H4 (H4Kac pan). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3632.00 USD - 9100.00 USD
PerkinElmer

LANCE Ultra ULight-p70 S6K (Thr389) Peptide

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Thr389 of human p70 S6K. The ULight-p70 S6K peptide can be phosphorylated at Thr389 by several kinases including mTOR, COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1, and TAOK2. Phosphorylation motif: LGFTYVAP
Price Range : 306.00 USD - 1250.00 USD
LANCE Detection Kit

Kappa Light Chain (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Kappa Light Chain Detection Kit is designed for detection and quantitation of human kappa light chain in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 930.00 USD - 12400.00 USD
Protein

ApoE (human), lyophilized (LANCE)

Human ApoE standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra ApoE Detection Kit, but can also be ordered separately.
List Price : 308.00 USD

IL-8 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-8 Detection Kit is designed for detection and quantitation of human interleukin 8 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 830.00 USD - 11000.00 USD
LANCE Detection Kit

Fibronectin (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Fibronectin Detection Kit is designed for detection and quantitation of human fibronectin in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 830.00 USD - 11100.00 USD
LANCE Detection Kit

IL17F (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-17F Detection Kit is designed for detection and quantitation of human interleukin 17 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 830.00 USD - 11100.00 USD
LANCE Eu-W1024 Anti-6xHis

LANCE Eu-W1024 Anti-6xHis

Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.
Price Range : 246.00 USD - 11600.00 USD
LANCE Detection Kit

PTEN (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human PTEN Detection Kit is designed for detection and quantitation of human PTEN in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 830.00 USD - 11100.00 USD
LANCE Detection Kit

CCL4 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human CCL4/MIP-1ß Detection Kit is designed for detection and quantitation of human C-C Motif Chemokine 4/Macrophage Inflammatory Protein-1beta in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 830.00 USD - 11100.00 USD
PerkinElmer

LANCE Ultra TREM2 Positive Control Supernatant

Control supernatant for LANCE Ultra TR-FRET TREM2 detection assay.
List Price : 365.00 USD

LANCE Ultra ULight™-eIF4E-binding protein 1 (Thr37/46) Peptide

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Synthetic peptide containing residues surrounding Thr37 and Thr46 of human eukaryotic translation initiation factor 4E-binding protein 1; phosphorylation sites: Thr37 and Thr46. The ULight-eIF4E-binding protein 1 peptide can be phosphorylated at Thr37/46 by mTOR and many kinases. Phosphorylation motif: STTPGGTLFSTTPG
Price Range : 417.00 USD - 899.00 USD

IL-1RA (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-1RA Detection Kit is designed for detection and quantitation of human interleukin-1RA in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 930.00 USD - 12400.00 USD
Protein

GLP-1 (7-36) (human), lyophilized (LANCE)

Human GLP-1 (7-36) standard, lyophilized, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human GLP-1 (7-36) TR-FRET Detection Kit, but can be ordered separately.
List Price : 250.00 USD
PerkinElmer

LANCE Ultra ERK1/2 Positive Control Lysate

Control lysates for LANCE Ultra TR-FRET cellular phospho-ERK and Total ERK detection assays.
List Price : 365.00 USD
Protein

IgA (human), lyophilized (LANCE)

Human IgA standard, for use in running standard curves in LANCE® Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human IgA Detection Kit, but can also be ordered separately.
List Price : 421.00 USD

Insulin (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Insulin Detection Kit is designed for detection and quantitation of human insulin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 830.00 USD - 11100.00 USD

IL-17A (mouse/rat) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse/Rat IL-17A Detection Kit is designed for detection and quantitation of mouse or rat interleukin 17A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 830.00 USD - 11100.00 USD
PerkinElmer

IL-8 (human), lyophilized (LANCE)

Human IL-8 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human IL-8 Detection Kit, but can also be ordered separately.
List Price : 258.00 USD
LANCE Detection Kit

c-Myc (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra c-Myc Detection Kit is designed for detection and quantitation of c-Myc peptide tag in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 830.00 USD - 11100.00 USD
PerkinElmer

Biotinylated human FCGR3A (176Phe) (LANCE)

Biotinylated human FCGR3A/CD16a(176Phe/F158) for use in LANCE Ultra TR-FRET Fc-receptor binding assays.
List Price : 308.00 USD
LANCE Detection Kit

ApoA1 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human ApoA1 Detection Kit is designed for detection and quantitation of human Apolipoprotein A1 (ApoA1) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 806.00 USD - 10700.00 USD
LANCE Detection Kit

E.coli HCP LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra E. coli Host Cell Proteins Detection Kit is designed for detection and quantitation of E.coli HCP in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 830.00 USD - 11100.00 USD
Protein

IL-12/p40 (human), lyophilized (LANCE)

Human IL-12/p40 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human IL-12/p40 Detection Kit, but can also be ordered separately.
List Price : 250.00 USD
LANCE Detection Kit

IL-2 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-2 Detection Kit is designed for detection and quantitation of human IL-2 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay. It is a fast and simple alternative to a human IL-2 ELISA kit.
Price Range : 830.00 USD - 11000.00 USD

ULight Anti-C-tag

ULight™ labeled anti-C-tag antibodies for LANCE® TR-FRET assays. This mouse monoclonal IgG1 antibody recognizes N-terminal and C-terminal Protein C tagged fusion proteins. The anti-C-tag antibody can be used to capture C-tag proteins, and can be used in conjunction with LANCE Europium-labeled reagents to create LANCE TR-FRET assays.
Price Range : 453.00 USD - 11100.00 USD
LANCE Detection Kit

CXCL10a (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human CXCL10 Detection Kit is designed for detection and quantitation of human CXCL10 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 829.00 USD - 11100.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total ERK 1/2 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra Total ERK 1/2 kits are designed for the detection of total (phosphorylated and non-phosphorylated) ERK1 and ERK2 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-ERK detection, and is compatible with both adherent and suspension cells.
Price Range : 1512.00 USD - 6800.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-AMPKα (Thr172) LANCE Ultra TR-FRET Detection Kit

LANCE Ultra phospho-AMPKα (Thr172) kits are designed for the detection of phosphorylated AMPKα in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1512.00 USD - 6800.00 USD

LANCE Ultra Europium-anti-O-linked-GlcNAc Antibody

Europium-labeled monoclonal antibody recognizing O-linked n-acetylglucosamine (O-linked GlcNAc). For use in LANCE® Ultra post-translational modification assays.
Price Range : 3632.00 USD - 9100.00 USD
PerkinElmer

LANCE Eu-W1024 Anti-HA

Europium-labeled anti-HA antibody, for capturing HA-tagged proteins and peptides in LANCE® TR-FRET assays.
Price Range : 1087.00 USD - 14500.00 USD
Protein

IFN-λ2 (human), lyophilized (LANCE)

Mouse IFN-λ2 standard, for use in running standard curves in LANCE® Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Mouse IFN-λ2 Detection Kit, but can also be ordered separately.
List Price : 782.00 USD

LANCE Ultra ULight-TK peptide

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing 28 amino acid peptide with 8 Tyrosine residues in different amino acid context that broadly provides for the recognition specificity of greater than 85% of 83 tested cytoplasmic and receptor tyrosine kinases.
Price Range : 407.00 USD - 1201.00 USD
LANCE Detection Kit

IL-17A (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-17A Detection Kit is designed for detection and quantitation of human IL-17A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 830.00 USD - 11000.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total AKT1 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total AKT1 kits are designed for the detection of total AKT1 (phosphorylated and unphosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-AKT1, and is compatible with both adherent and suspension cells.
Price Range : 1512.00 USD - 6800.00 USD
Protein

MMP-12 (human), lyophilized (LANCE)

Human MMP-12 standard, lyophilized, for use in running standard curves in LANCE TR-FRET assays. This standard is already provided in the LANCE Ultra MMP-12 TR-FRET Detection Kit, but can be ordered separately.
List Price : 317.00 USD

LANCE Ultra Europium-anti-methyl-Histone H3/H4 Arginine (H3/H4Rme pan) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 and H4 methylated arginine residues. For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3632.00 USD - 9100.00 USD
Protein

IGF-1 (human), lyophilized (LANCE)

Human IGF-1 standard, lyophilized, for use in running standard curves in LANCE TR-FRET assays. This standard is already provided in the LANCE Ultra IGF-1 TR-FRET Detection Kit, but can be ordered separately.

 

List Price : 258.00 USD
LANCE Detection Kit

PCSK9 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human PCSK9 Detection Kit is designed for detection and quantitation of human Proprotein Convertase Subtilisin/Kexin Type 9 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 829.00 USD - 11100.00 USD

"LANCE TR-FRET" 1-50 of 363 Products & Services

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