PerkinElmer

LANCE TR-FRET

OF

LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

Assays for Biomarker Detection
Assays for Biomarker Detection

Assays for Biomarker Detection

Biomarker detection is key for advancing precision medicine. An array of biomarker detection kits are available utilizing our LANCE technologies in therapeutic areas including:
  • Angiogenesis
  • Animal Health
  • Autophagy
  • Biologics/Bioprocess
  • Cancer
  • Cardiovascular
  • Central nervous system
  • Inflammation
  • Metabolic
OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

iStock
iStock

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. http://www.perkinelmer.com/LANCE/ASK.

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1-25 of 349 Products & Services

  • Protein

    C-peptide (mouse), lyophilized (LANCE)

    Mouse C-peptide standard, lyophilized, for use in running standard curves in LANCE TR-FRET assays. This standard is already provided in the LANCE Ultra C-peptide TR-FRET Detection Kit, but can be ordered separately.

     

    List Price : 575.00 USD
  • LANCE Ultra Europium-anti-acetyl-Histone H3 Lysine 27 (H3K27ac) Antibody

    Europium-labeled monoclonal antibody recognizing human Histone H3 acetylated at lysine 27 (H3K27ac). For use in LANCE® Ultra epigenetic writer and eraser assays.
    Price Range : 3787.00 USD - 9500.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

    Phospho-ZAP-70 (Tyr319) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-ZAP-70 (Tyr319) kits are designed for the detection of phosphorylated ZAP-70 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • LANCE Detection Kit

    TNFα (mouse) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Mouse TNFα Detection Kit is designed for detection and quantitation of mouse tumor necrosis factor α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra ULight-TK peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing 28 amino acid peptide with 8 Tyrosine residues in different amino acid context that broadly provides for the recognition specificity of greater than 85% of 83 tested cytoplasmic and receptor tyrosine kinases.
    Price Range : 425.00 USD - 1252.00 USD
  • LANCE Detection Kit

    MANF (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human MANF Detection Kit is designed for detection and quantitation of human MANF in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 840.00 USD - 11200.00 USD
  • LANCE Detection Kit

    CCL2/MCP-1α (mouse) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Mouse CCL2/MCP-1α Detection Kit is designed for detection and quantitation of mouse Monocyte Chemoattractant Protein 1α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

    Phospho-NFκB (Ser536) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-NFκB (Ser536) kits are designed for the detection of phosphorylated NFκB in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

    Total NFκB LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra total NFκB kits are designed for the detection of NFκB (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-NFκB assay.
    Price Range : 1557.00 USD - 7000.00 USD
  • LANCE Detection Kit

    ANGPTL4 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human ANGPTL4 Detection Kit is designed for detection and quantitation of human angiopoietin-like 4 protein in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

    Phospho-SLP-76 (Ser376) LANCE Ultra TR-FRET Cellular Detection Kit

    LANCE Ultra phospho-SLP-76 (Ser376) kits are designed for the detection of SLP76 (phosphorylated at S376) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • LANCE Detection Kit

    IgG (bovine) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Bovine IgG Detection Kit is designed for detection and quantitation of bovine IgG in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 1557.00 USD - 10300.00 USD
  • LANCE Ultra ULight™-Crosstide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Crosstide peptide (sequence: CGSGSGRPRTSSFAEG) labeled with ULight dye. Synthetic peptide derived from glycogen synthase kinase-3 alpha (GSK-3α) (Phosphorylation site: Ser21). GSK-3α can be phosphorylated by a variety of kinases including AKT1, AKT2, AKT3, MSK1, AMS2, PDK1, PLK3.
    Price Range : 344.00 USD - 938.00 USD
  • LANCE Detection Kit

    TSH (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human TSH Detection Kit is designed for detection and quantitation of human thyroid stimulating hormone in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Eu-W1024-ITC Chelate and Eu Standard, 0.2 mg

    Europium labeling chelate for creating your own Europium-labeled biomolecules for use in LANCE TR-FRET assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.
    List Price : 2834.00 USD
  • PerkinElmer

    SureLight Allophycocyanin-streptavidin (APC-SA), protein binding assay, Iyophilized

    APC-labeled streptavidin, for use in LANCE® TR-FRET protein binding assays.
    Price Range : 432.00 USD - 12100.00 USD
  • LANCE Detection Kit

    IL-4 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-4 Detection Kit is designed for detection and quantitation of human interleukin 4 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-W1024 Anti-Human IgG

    Europium-labeled anti-human IgG, for capturing antibodies in LANCE® TR-FRET assays.
    Price Range : 229.00 USD - 9900.00 USD
  • LANCE standard

    IFN-γ (mouse), lyophilized (LANCE)

    Mouse IFNγ standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Mouse IFNγ Detection Kit, but can also be ordered separately.
    List Price : 269.00 USD
  • LANCE Detection Kit

    IGF-1 (rat) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Rat IGF-1 Detection Kit is designed for detection and quantitation of rat insulin-like growth factor 1 (IGF-1) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 840.00 USD - 11200.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra NFκB Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-NFκB (Ser536) and Total NFκB detection assays.
    List Price : 376.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra BTK Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-BTK and Total BTK detection assays.
    List Price : 376.00 USD
  • AlphaLisa Analyte 2

    MANF (human), lyophilized (LANCE)

    Human MANF standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human MANF TR-FRET Detection Kit, but can also be ordered separately.
    List Price : 253.00 USD
  • LANCE Detection Kit

    Chorionic gonadotropin (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Chorionic Gonadotropin (hCG) Detection Kit is designed for detection and quantitation of hCG in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 840.00 USD - 11200.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

    Total ERK 1/2 LANCE Ultra TR-FRET Cellular Detection Kit

    LANCE Ultra Total ERK 1/2 kits are designed for the detection of total (phosphorylated and non-phosphorylated) ERK1 and ERK2 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-ERK detection, and is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
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