PerkinElmer

LANCE TR-FRET

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LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format. We now also offer PerkinElmer Cisbio HTRF® assay technology. HTRF (Homogeneous Time Resolved Technology), allowing us to provide a wide range of assays kits in a homogenous TR-FRET technology.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

HTRF_84458_320x320f
HTRF_84458_320x320f

HTRF (Homogeneous Time Resolved Technology)

 

We now also offer PerkinElmer Cisbio HTRF® assay technology. Find out more about HTRF (Homogeneous Time Resolved Technology) and the products offered in this technology here.

Assays for Biomarker Detection

Biomarker detection is key for advancing precision medicine. An array of biomarker detection kits are available utilizing our LANCE technologies in therapeutic areas including:
  • Angiogenesis
  • Animal Health
  • Autophagy
  • Biologics/Bioprocess
  • Cancer
  • Cardiovascular
  • Central nervous system
  • Inflammation
  • Metabolic
OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

iStock
iStock

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. https://www.perkinelmer.com/LANCE/ASK.

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1-25 of 335 Products & Services

  • ULight anti-human IgG

    ULight™ labeled anti-human IgG antibody for LANCE® TR-FRET assays. The anti-human IgG antibody is a purified goat polyclonal antibody, and reacts with whole molecule human IgG.
    Price Range : 486.00 USD - 12300.00 USD
  • LANCE Ultra ULight™-poly GAT

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Poly Glu-Ala-Tyr (1:1:1) labeled with ULight dye. This random polymer is a Tyrosine kinase substrate.
    Price Range : 475.00 USD - 1087.00 USD
  • LANCE Ultra Europium-anti-phospho-Myelin Basic Protein (MBP)

    Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Thr232 in myelin basic protein (MBP; Swiss-Prot: P02686). MBP can be phosphorylated by a variety of kinases including Erk1/Erk2. Sequence motif recognized: FFKNIVTPRpTPPPSQGK
    Price Range : 333.00 USD - 6800.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra TBK1 Positive Control Lysate

    Control lysate for LANCE Ultra TR-FRET cellular phospho-TBK1 detection assays.
    List Price : 392.00 USD
  • LANCE Ultra ULight™-phospho-40S ribosomal protein S6 (pSer235/236) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Synthetic peptide containing residues surrounding Ser235 and Ser236 of human 40S ribosomal protein S6; this peptide is pre-phosphorylated on Ser235; phosphorylation site: Ser236. This peptide is phosphorylated at Ser236 efficiently by kinases from the RSK family. Phosphorylation motif: RRL(pS)SLRA
    Price Range : 562.00 USD - 998.00 USD
  • LANCE standard

    LANCE Ultra hIL-11, lyophilized (0.3ug)

    Human IL-11 standard, for use in running standard curves in LANCE® Ultra TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human IL-11 Detection Kit, but can be ordered separately.
    List Price : 278.00 USD
  • PerkinElmer

    SureLight Allophycocyanin-anti-GST antibody, 1 mg, lyophilized

    APC-labeled anti-GST antibody, for capturing GST-tagged proteins and peptides in LANCE TR-FRET assays.
    List Price : 564.00 USD
  • PerkinElmer

    TNFα (human), lyophilized (LANCE)

    Human TNFα standard, lyophilized, for use in running standard curves in LANCE TR-FRET detection assays. This standard is already provided in the LANCE Ultra Human TNFα Detection Kit, but can be ordered separately.
    List Price : 273.00 USD
  • LANCE Ultra Europium-anti-phospho-CREB (Ser133)

    Monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser133 in the cAMP response element binding (CREB) protein. CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
    Price Range : 1264.00 USD - 5400.00 USD
  • LANCE Ultra Biomarker

    IL-17A (mouse/rat) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra™ Mouse/Rat IL-17A Detection Kit is designed for detection and quantitation of mouse or rat interleukin-17A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

    This is a replacement for TRF1514.

    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra ULight-anti-6xHis

    Mouse monoclonal anti-polyHistidine tag antibody labeled with ULight™ dye.

    This is a replacement for TRF0105.

    Price Range : 509.00 USD - 12600.00 USD
  • LANCE Ultra Biomarker

    EGF-EGFR LANCE Ultra TR-FRET Binding Kit

    This kit is designed for the detection of binding activity between Human EGF and EGFR using a homogeneous LANCE Ultra TR-FRET assay. This assay can facilitate the design and development of therapeutics which competitively inhibit EGF/EGFR interaction.
    Price Range : 877.00 USD - 11700.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-W1024 Anti-c-Myc

    Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.
    Price Range : 338.00 USD - 16300.00 USD
  • LANCE Ultra Biomarker

    Residual Protein A LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Residual Protein A Detection Kit is designed for detection and quantitation of Residual Protein A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra SMAD2 Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-SMAD2 detection assays.
    List Price : 392.00 USD
  • PerkinElmer

    E-cadherin (human), lyophilized (LANCE)

    Human E-cadherin standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human E-cadherin TR-FRET Detection Kit, but can also be ordered separately.
    List Price : 335.00 USD
  • PerkinElmer

    COL1A1 (human), lyophilized (LANCE)

    Human COL1A1 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human COL1A1 TR-FRET Detection Kit, but can also be ordered separately.
    List Price : 335.00 USD
  • LANCE Ultra ULight™-IkappaB-alpha (Ser32/36) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser32/36 of human IkappaB-alpha. The ULight-IkappaB-alpha peptide can be phosphorylated at Ser 32/36 by the IKKβ kinase. Peptide core motif: RHDSGLDSM
    Price Range : 356.00 USD - 969.00 USD
  • LANCE Ultra ULight™-poly GT

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Poly Glu-Tyr (4:1) labeled with ULightTM dye. This random polymer is a Tyrosine kinase substrate.
    Price Range : 359.00 USD - 979.00 USD
  • PerkinElmer

    PAI-1 (human), lyophilized (LANCE)

    Human PAI-1 standard, lyophilized, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human PAI-1 TR-FRET Detection Kit, but can be ordered separately.
    List Price : 273.00 USD
  • LANCE Ultra Biomarker

    ApoA1 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human ApoA1 Detection Kit is designed for detection and quantitation of human Apolipoprotein A1 (ApoA1) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 877.00 USD - 11700.00 USD
  • LANCE Ultra Biomarker

    Total NFκB LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra total NFκB kits are designed for the detection of NFκB (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-NFκB assay.
    Price Range : 1626.00 USD - 7300.00 USD
  • LANCE Ultra Europium-anti-di-methyl-Histone H3 Lysine 36 (H3K36me2) Antibody

    Europium-labeled monoclonal antibody recognizing human Histone H3 di-methylated at lysine 36 (H3K36me2). For use in LANCE® Ultra epigenetic writer and eraser assays.
    Price Range : 4031.00 USD - 9900.00 USD
  • LANCE Ultra Biomarker

    ANGPTL3 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human ANGPTL3 Detection Kit is designed for detection and quantitation of human angiopoietin-like 3 protein in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra Biomarker

    PD-1 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human PD-1 Detection Kit is designed for detection and quantitation of human PD-1 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 877.00 USD - 11700.00 USD
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