PerkinElmer

Kinase

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Study phosphorylation of protein, peptide, and lipid substrates for kinase inhibition, screening, and mechanism-of-action studies. We offer a range of end-point and real-time kinetic assay kits, technologies, and instruments such as:

  • Labeled substrates for kinetic assays and antibody-free screening
  • Alpha kits for highly-sensitive detection of cellular phosphorylation
  • Fast and simple TR-FRET assays using pre-validated peptide and protein substrates derived from biologically-relevant targets
  • Microfluidic mobility-shift assays (MSA) for ratiometric analysis of substrate conversion
  • Multi-mode plate readers for maximal speed and throughput independent of technology platform

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  • Phosphoprotein (129)
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  • Microplates (176)
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  • alpha (1)
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  • 40-50 (2)
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  • Phosphorylation (119)
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  • Alpha SureFire Ultra Multiplex (8)
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Radionuclide
  • 32P (9)
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Radioisotope
  • P-32 (9)
  • P-33 (2)
  • S-35 (5)
Sample Type
  • Enzyme (35)
Target Species
  • Human (129)

"Kinase" 1-50 of 271 Products and Services

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EnSight multimode plate-reader

EnSight Multimode Plate Reader

The EnSight multimode plate reader combines labeled, label-free* & image-based cytometry technologies in a single benchtop platform. Imaging & online data analysis is fast: a 384-well plate can be imaged in less than 5 mins. The system’s modular design, combined with workflow-based Kaleido data acquisition & analysis software, gives a truly versatile plate reader that gets users productive quickly.

*Label free detection module of EnSight Plate Reader is available outside the EU. Please contact your local representative for further details.

AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-mTOR (Ser2448) HV Assay Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha no-wash technology.
List Price : 475.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-ERK 1/2 (Thr202/Tyr204) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-ERK 1/2 (Thr202/Tyr204) kits are designed for the detection of phosphorylated ERK 1 and ERK2 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1450.00 USD - 6500.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-mTOR (Ser2448) Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha no-wash technology.
List Price : 27600.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-AKT 1/2/3 (Ser473) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-AKT1/2/3 (Ser473) kits are designed for the detection of AKT1, AKT2, and AKT3 (phosphorylated at residue S473) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1450.00 USD - 6500.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-eIF2α (Ser51) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-eIF2α (Ser51) assay is a sandwich immunoassay for quantitative detection of phospho-eIF2α (phosphorylated on Ser51) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD

LANCE Ultra ULight™-MBP peptide

MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight™ dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.
Price Range : 307.00 USD - 838.00 USD

LANCE Ultra ULight-Glycogen Synthase (Ser641/pSer657) Peptide

Glycogen synthase peptide (Sequence: PASVPPSPSLSRHSSPHQ(pS)ED) labeled with ULight™ dye. Synthetic peptide containing residues surrounding Ser641 and Ser657 of human Muscle Glycogen Synthase. This peptide is pre-phosphorylated on Ser657. Target phosphorylation site: Ser641. This peptide substrate can be phosphorylated by a variety of kinases including GSK3α and GSK3β.
Price Range : 381.00 USD - 837.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT6 (Tyr641) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT6 (Tyr641) assay is a sandwich immunoassay for quantitative detection of phospho-STAT6 (phosphorylated on Tyr641) in cellular lysates using Alpha Technology.
Price Range : 461.00 USD - 26800.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-NFκB (Ser536) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-NFκB (Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NFκB (phosphorylated on Ser536) in cellular lysates using Alpha Technology.
List Price : 8800.00 USD

LANCE Ultra ULight™-Crosstide

Crosstide peptide (sequence: CGSGSGRPRTSSFAEG) labeled with ULight™ dye. Synthetic peptide derived from glycogen synthase kinase-3 alpha (GSK-3α) (Phosphorylation site: Ser21). GSK-3α can be phosphorylated by a variety of kinases including AKT1, AKT2, AKT3, MSK1, AMS2, PDK1, PLK3.
Price Range : 307.00 USD - 838.00 USD
SureFire principle

AlphaScreen SureFire CHK2 (p-Thr68) Assay Kit 

The AlphaScreen® SureFire® p-CHK2 (Thr68) assay is a sandwich immunoassay for quantitative detection of phospho-CHK2 (phosphorylated on Thr68 in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
SureFire principle

AlphaScreen SureFire EGFR (p-Tyr1068) Assay Kit

The AlphaScreen® SureFire® p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGFR (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology.
Price Range : 1590.00 USD - 17200.00 USD
PerkinElmer

ProfilerPro Kinase Peptide Substrate 14, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 14 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 721.00 USD
PerkinElmer

LANCE Ultra ULight-p70 S6K (Thr389) Peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Thr389 of human p70 S6K. The ULight-p70 S6K peptide can be phosphorylated at Thr389 by several kinases including mTOR, COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1, and TAOK2. Phosphorylation motif: LGFTYVAP
Price Range : 285.00 USD - 1164.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-STAT3 (Tyr705) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-STAT3 (Tyr705) kits are designed for the detection of phosphorylated STAT3 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1450.00 USD - 6500.00 USD
Alpha SureFire Ultra Multiplex kits

Alpha SureFire Ultra Multiplex p-AKT(Ser473) (Eu) + p-ERK (Tb) Kit

The Alpha SureFire® Ultra Multiplex kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of another protein, phospho or total. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm).
Price Range : 855.00 USD - 49600.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SLP-76 (Ser376) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.
List Price : 1605.00 USD
alphascreen surefire total

AlphaScreen SureFire Monoclonal Total ERK, 500 Assay Points

The AlphaScreen® SureFire® total ERK assay is a sandwich immunoassay for quantitative detection of ERK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. This assay performs similarly to the TGRTES SureFire kit, but contains a monoclonal antibody for ERK detection.
List Price : 1590.00 USD
PerkinElmer

LANCE Ultra ULight™-CREBtide

CREBtide peptide (sequence: CKRREILSRRPSYRK) labeled with ULight™ dye. Synthetic peptide derived from human cAMP Response Element Binding (CREB) Protein (phosphorylation site: Ser133). CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
Price Range : 231.00 USD - 838.00 USD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

DELFIA Eu-N1 Anti-Phosphtyrosine (P-Tyr-100)

Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in DELFIA® time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.
Price Range : 234.00 USD - 3143.00 USD

TPγS, [35S]- 25-100Ci/mmol, 12.5mCi/ml, 1/EA

Adenosine triphosphate, labeled on the gamma phosphate group with 35S (ATPγS), is used in protein kinase assays (thiosphosphorylation) in place of ATP
Price Range : 839.00 USD - 1596.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Insulin Receptor β (Tyr1150/1151) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-Insulin Receptor β (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-INSR (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total SLP-76 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total SLP-76 kits are designed for the detection of SLP-76 (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-SLP-76, and is compatible with both adherent and suspension cells.
SureFire principle

AlphaScreen SureFire ELK1 (p-Ser383) Assay Kit

The AlphaScreen® SureFire® p-ELK1 (Ser383) assay is a sandwich immunoassay for quantitative detection of phospho-ELK1 (phosphorylated on Ser383) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
PerkinElmer

ProfilerPro Kinase Peptide Substrate 19, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 19 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 721.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-Crosstide (GSK-3α Ser21)

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser21 in glycogen synthase kinase-3 alpha (GSK-3α), Crosstide and other peptides derived from GSK-3α. GSK-3α can be phosphorylated by a variety of kinases including AKT1, AKT2, AKT3, MSK1, AMS2, PDK1, PLK3. Sequence motif recognized: GRPRTSpSFAEG
Price Range : 1802.00 USD - 5800.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-CREB (Ser133)

Monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser133 in the cAMP response element binding (CREB) protein. CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
Price Range : 1080.00 USD - 4677.00 USD
SureFire principle

AlphaScreen SureFire MKK4 (p-Ser257;Thr261) Assay Kit

The AlphaScreen® SureFire® p-MKK4 (Ser257/Thr261) assay is a sandwich immunoassay for quantitative detection of phospho-MKK4 (phosphorylated on Ser257/Thr261) in cellular lysates using Alpha Technology.
Price Range : 1448.00 USD - 15600.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total EGFR LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total EGFR kits are designed for the detection of EGFR (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-EGFR, and is compatible with both adherent and suspension cells.
Price Range : 1450.00 USD - 6500.00 USD
SureFire principle

AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD

ATP, [γ-32P]- 3000Ci/mmol 5mCi/ml EasyTide Lead

Adenosine triphosphate, labeled on the gamma phosphate group with 32P.
Price Range : 443.00 USD - 659.00 USD
PerkinElmer

LANCE® Eu-W1024 Anti-phosphotyrosine (PT66)

Europium-labeled anti-phosphotyrosine (PT66) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 1013.00 USD - 8900.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-STAT5 (Tyr694/699) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-STAT5 (Tyr694/699) kits are designed for the detection of STAT5 (phosphorylated at Y694/Y699) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1450.00 USD - 6500.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-4E-BP1 (Thr37/46) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-4E-BP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ Multiplex p-ERK (Eu) (directly coupled) Kit, 500 Assay Points

Single target-only kit for multiplexing to other AlphaLISA SureFire Ultra assay kits. The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of another protein, phospho or total. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm).
List Price : 1605.00 USD
SureFire principle

AlphaScreen SureFire ATF1 (p-Ser63) Assay Kit

The AlphaScreen® SureFire® p-ATF1 (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-ATF1 (phosphorylated on Ser63) in cellular lysates using Alpha Technology.
Price Range : 1448.00 USD - 15600.00 USD

ATP, [γ-32P]- 3000Ci/mmol 5mCi/ml , 250 µCi

Adenosine triphosphate, labeled on the gamma phosphate group with 32P
List Price : 424.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-eIF4E (Ser209) Assay Kits

The AlphaLISA® SureFire® Ultra™ p-eIF4E(Ser209) assay is a sandwich immunoassay for quantitative detection of phospho-eIF4E (phosphorylated on Ser209) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD
SureFire principle

AlphaScreen SureFire Phospho-MEK 1 Kit

The AlphaScreen® SureFire® p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit 

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
SureFire principle

AlphaScreen SureFire STAT3 (p-Tyr705) Assay Kit

The AlphaScreen® SureFire® p-STAT3 (p-Ser705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Ser705) in cellular lysates using Alpha Technology.
Price Range : 435.00 USD - 17200.00 USD

LANCE Ultra ULight™-PKC substrate

PKC substrate (sequence: CRFARKGSLRQKNV) labeled with ULight™ dye. Synthetic peptide derived from amino acids 19-31 of PKC?.
Price Range : 307.00 USD - 838.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium chelate. This antibody detects phospho-Ser in a synthetic peptide (RFARKGSLRQKNV) only after the peptide is phosphorylated by PKC or PKA kinases. The synthetic peptide is derived from amino acids 19-31 of human PKCα in which Ala25 is mutated to Ser. Sequence motif recognized: RFARKG-pS-LRQKNV
Price Range : 1984.00 USD - 6800.00 USD
SureFire principle

AlphaScreen SureFire Phospho-GSK 3a (Ser21) Kit

The AlphaScreen® SureFire® p-GSK 3α (Ser21) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3α (phosphorylated on Ser21) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total STAT3 HV Assay Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ Total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
List Price : 475.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Thr1343) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II alpha (p-Thr1343) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II alpha (phosphorylated on Thr1343) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total SLP-76 Assay Kit

The AlphaLISA® SureFire® Ultra Total SLP-76 assay is a sandwich immunoassay for quantitative detection of SLP-76 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 461.00 USD - 26800.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SYK (Tyr525/526) Assay Kit

The AlphaLISA® SureFire® Ultra p-SYK (Tyr525/526) assay is a sandwich immunoassay for quantitative detection of phospho-SYK (phosphorylated on Tyr525/526) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27600.00 USD

"Kinase" 1-50 of 271 Products and Services

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