PerkinElmer

In Situ Hybridization

OF

Reliably detect and quantitate many nucleic acids that cannot be measured with standard methods using in situ hybridization. Our reagent kits are widely cited for detection of DNA, mRNA and miRNA sequences, with some researchers reporting detection of single copies.

Signal amplification with our kits leads to reliable results, even with short synthetic oligonucleotide probes. These can be used with any hapten labeled probe for fluorescent or chromogenic signal generation.

Advantages of using In Situ Hybridization:

  • Improved limit of detection and quantitative range
  • Reduced exposure time for faster imaging
  • Excellent localization of signal to the target

Tumor Microenvironment Opal 7 Color Image
Tumor Microenvironment Opal 7 Color Image

Stain

Simultaneously detect eight biomarkers plus DAPI within a single tissue section. Similar to standard immunohistochemistry, our advanced method is accessible to many laboratories where IHC method development is performed. It allows researchers to use the best primary antibodies, even those raised in the same species, together for multiplex detection. Our staining solutions provide researchers with tools to assess multiple cellular phenotypes at the same time while retaining context provided within tissue sections
Vectra® Polaris™ Automated Quantitative Pathology Imaging System
Vectra® Polaris™ Automated Quantitative Pathology Imaging System

Image

Our quantitative pathology imaging systems deliver high quality data where morphological context is preserved down to the sub-cellular level, removing autofluorescence and increasing signal-to-noise. These systems detect and measure multiple biomarkers, even if overlapping, within a single IHC or IF tissue section or TMA. Low signal levels that were previously undetected can now be captured, and overall quantification is vastly improved - particularly for solid, formalin-fixed, and paraffin-embedded tissues.
inform Image Analysis Software for Digital Pathology
inform Image Analysis Software for Digital Pathology

Analyze

Our software programs combine the latest technologies with algorithms and intuitive, easy-to-use interfaces, giving you the power to make new, exciting discoveries from your data. Our automated advanced image analysis software allows you to visualize, analyze, quantify and phenotype cells labeled with multiple biomarkers in situ in FFPE tissue sections.
Contract Research Services for Phenotyping In Situ
Contract Research Services for Phenotyping In Situ

Contract Research Services

Interested in testing the Phenoptics™ workflow? Our Research Services team can perform multiplexed IHC for up to seven colors (six markers plus DAPI) to help you get results.
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1-12 of 102 Products & Services

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  • TSA Plus Cyanine 5/Fluorescein System

    TSA Plus Cyanine 5 and Flourescein System

    TSA® Plus multi-fluorophore detection kit, for amplification of multiple signals in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols.
    List Price : 708.00 USD
  • TSA Cyanine 3/Fluorescein System

    TSA Plus Cyanine 3 and Fluorescein System

    TSA® Plus multi-fluorophore detection kit, for amplification of multiple signals in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols.
    List Price : 708.00 USD
  • PerkinElmer

    TSA Biotin Reagent Pack

    TSA® biotin reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
    Price Range : 857.00 USD - 3964.00 USD
  • Fluorescein-12-ATP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 347.00 USD
  • PerkinElmer

    Cyanine 5-dUTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 489.00 USD
  • PerkinElmer

    TSA Plus Fluorescein

    TSA® Plus fluorescein detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra fluorescein in the localized area of the probe or antibody.
    Price Range : 223.00 USD - 1779.00 USD
  • PerkinElmer

    TAMRA-ddUTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 474.00 USD
  • PerkinElmer

    TSA Plus Biotin

    TSA® Plus Biotin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra biotin in the localized area of the probe or antibody.
    Price Range : 383.00 USD - 1436.00 USD
  • TSA Biotin System

    TSA Biotin System

    TSA® biotin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra biotin in the localized area of the probe or antibody.
    Price Range : 319.00 USD - 1063.00 USD
  • Amplification Diluent

    TSA Cyanine 5 Tyramide Reagent Pack, Reagents for 50-150 Slides

    TSA® Cyanine 5 (Cy5) reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
    List Price : 330.00 USD
  • Fluorescein-12-CTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 354.00 USD
  • PerkinElmer

    TSA Plus Cyanine 3 System, for 50-150 Slides

    TSA® Plus Cyanine 3 (Cy3) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3 in the localized area of the probe or antibody.
    List Price : 406.00 USD
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