PerkinElmer

LANCE TR-FRET Immunoassays

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ELISA, western blot, and other immunodetection technologies have commonly been used for quantitation of biomarkers and other analytes in a variety of sample types. These methods necessitate long, tedious protocols and often provide limited detectable range.

LANCE® (Lanthanide Chelate Excite) TR-FRET is a homogeneous (no-wash) alternative to traditional ELISA. LANCE is a proximity assay that combines the benefits of time resolution (TR) with fluorescence resonance energy transfer (FRET) principles by using energy donor and energy acceptor fluorophore labels. It’s everything you love about TRF detection with faster time to results and no wash steps.

 

In a LANCE TR-FRET immunoassay, a target specific antibody is either conjugated directly to or is captured by an antibody conjugated to a Europium chelate. A second, target specific, antibody is either conjugated to or is captured by an antibody conjugated to the ULight™ acceptor dye. These two target- specific antibody capture the analyte, brining the Europium chelate donor and the ULight acceptor dye into proximity. Upon excitation at 320 nm or 340 nm, the Europium chelate is excited and energy is transferred to the ULight dye, which emits light at 665 and 615 nm. The amount of light emitted is proportional to the amount of analyte present in the sample.

LANCE TR-FRET offers benefits over traditional ELISA and other immunodetection technologies including:

  • No-wash
  • Streamlined protocol with one or 2 additional steps
  • Faster time to results
  • Miniaturizable
  • Wider dynamic range, up to 4 log
  • Longer signal stability

For research use only. Not for use in diagnostic procedures.

 

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100x100_NewestAssays

Make the switch to LANCE TR-FRET

Switching to a new immunoassay platform may seem like a large undertaking, but it doesn’t have to be. We have a team of experts ready to help you make the switch. We have a wide range of support options from development guides to or Application Support Knowledgebase (ASK) and from technical support, to our global Field Application Scientist who are hands-on subject matters in assay development.
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OnPoint_2color_100x100

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.
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1-25 of 181 Products & Services

  • LANCE Ultra Biomarker

    Leptin (mouse) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Mouse Leptin Detection Kit is designed for detection and quantitation of mouse leptin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra Biomarker

    VEGF (mouse) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Mouse VEGF-A Detection Kit is designed for detection and quantitation of human CXCL1/GRO-α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra Biomarker

    MMP-10 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human MMP-10 Detection Kit is designed for detection and quantitation of human MMP-10 in cell culture media and cell supernatants using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 2507.00 USD - 11600.00 USD
  • LANCE Ultra Biomarker

    Leptin (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Leptin Detection Kit is designed for detection and quantitation of human leptin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra Biomarker

    EGFR (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human EGFR Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor Receptor in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra Biomarker

    IgG (mouse) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Mouse IgG Detection Kit is designed for detection and quantitation of Mouse IgG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra Biomarker

    IL-6 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra human IL-6 Detection Kit is designed for detection and quantitation of human interleukin-6 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • ULight anti-mouse IgG

    ULight™ labeled anti-mouse IgG antibody for LANCE® TR-FRET assays. The anti-mouse IgG antibody is a purified goat polyclonal antibody.
    Price Range : 465.00 USD - 11800.00 USD
  • LANCE Ultra Biomarker

    EGF (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human EGF Detection Kit is designed for detection and quantitation of human epidermal growth factor in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra Biomarker

    Tau (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human TAU Detection Kit is designed for detection and quantitation of human Tau in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra Biomarker

    CRP (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Human CRP Detection Kit is designed for detection and quantitation of human C-reactive protein in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • PerkinElmer

    MMP-10 (human), lyophilized (LANCE)

    Recombinant human MMP-10 standard, for use in LANCE Ultra TR-FRET quantitation assays. This standard is already included in the LANCE Ultra human MMP-10 detection kit, but can also be ordered separately.
    List Price : 791.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-W1024 Anti-c-Myc

    Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.
    Price Range : 324.00 USD - 15600.00 USD
  • LANCE Ultra Biomarker

    CCL4 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human CCL4/MIP-1ß Detection Kit is designed for detection and quantitation of human C-C Motif Chemokine 4/Macrophage Inflammatory Protein-1beta in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra Biomarker

    LC3B (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human LC3B Detection Kit is designed for detection and quantitation of human microtubule-associated protein 1 light chain 3 isoform B (LC3B) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra Biomarker

    ErbB2 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human ErbB2 (HER2) Detection Kit is designed for detection and quantitation of human ErbB2 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra Phopho-protein

    Phospho-SMAD3 (Ser423/425) LANCE Ultra TR-FRET Cellular Detection Kit

    LANCE Ultra phospho-SMAD3 (Ser423/425) kits are designed for the detection of SMAD3 (phosphorylated at residue S423/S425) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • ULight-anti-V5 (LANCE TR-FRET)

    Anti-V5 tag antibody labeled with ULight™ dye. This ULight-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® Europium-labeled reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
    Price Range : 451.00 USD - 11400.00 USD
  • LANCE Ultra Biomarker

    E cadherin (human) LANCE Ultra TR-FRET Detection Kit

    Human E-cadherin standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human E-cadherin TR-FRET Detection Kit, but can also be ordered separately.
    Price Range : 840.00 USD - 11200.00 USD
  • LANCE Ultra Biomarker

    MMP-8 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human MMP-8 Detection Kit is designed for detection and quantitation of human matrix metalloproteinase 8 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • ULight anti-rabbit IgG

    ULight™ labeled anti-rabbit IgG antibody for LANCE® TR-FRET assays. The anti-rabbit IgG antibody is a purified mouse polyclonal antibody.
    Price Range : 465.00 USD - 11800.00 USD
  • LANCE Ultra Biomarker

    ApoA1 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human ApoA1 Detection Kit is designed for detection and quantitation of human Apolipoprotein A1 (ApoA1) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 840.00 USD - 11200.00 USD
  • LANCE Ultra Biomarker

    FSH (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Follicle Stimulating Hormone (FSH) Detection Kit is designed for detection and quantitation of human FSH in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 840.00 USD - 11200.00 USD
  • LANCE Ultra Europium-anti-methyl-Histone H4 Arginine 3 (H4R3me) Antibody

    Europium-labeled monoclonal antibody recognizing human Histone H4 methylated at arginine3 (H4R3me). For use in LANCE® Ultra epigenetic writer and eraser assays.
    Price Range : 3787.00 USD - 9400.00 USD
  • LANCE Ultra Biomarker

    Frataxin (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Frataxin Detection Kit is designed for detection and quantitation of human frataxin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
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