PerkinElmer

AlphaLISA Immunoassays

OF

ELISA, western blot, and other immunodetection technologies have commonly been used for quantitation of biomarkers and other analytes in a variety of sample types. These methods necessitate long, tedious protocols and often provide limited detectable range.

AlphaLISA® provides a homogenous (no-wash) alternative to ELISAs, with a simple, streamlined workflow that can be used to detect and quantitate biomolecules in both simple and complex sample types. AlphaLISA is a bead-based luminescent amplification assay, offering greater sensitivity, a wider dynamic range and small sample sizes over traditional ELISA.

 

In the AlphaLISA assay, a biotinylated antibody and an antibody-conjugated AlphaLISA Acceptor bead are used to capture the target analyte. The biotinylated antibody associates with an Alpha streptavidin-coated Donor bead. When the analyte is present in the sample, the Donor and Acceptor beads are brought together. Upon excitation, a photosensitizer inside the Donor bead converts ambient oxygen to an excited singlet state. Singlet oxygen diffuses up to 200 nm to produce a chemiluminescent reaction in the Acceptor bead, leading to light emission. The amount of light is proportional to the amount of analyte present in your sample.

Alpha offers the benefits over ELISAs including:

  • No-wash
  • Faster time to results
  • Requires a fraction of the sample size, ~5µL
  • Wider dynamic range up to 5 log
  • Improved sensitivity
  • Ability to multiplex
  • Ability to study large protein complexes

For research use only. Not for use in diagnostic procedures.

 

Make the switch to AlphaLISA

Switching to a new immunoassay platform may seem like a large undertaking, but it doesn’t have to be. We have a team of experts ready to help you make the switch. We have a wide range of support options from development guides to or Application Support Knowledgebase (ASK) and from technical support, to our global Field Application Scientist who are hands-on subject matters in assay development.
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OnPoint_2color_100x100

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can custom conjugate your antibody or biomolecule to any bead, or biotinylate or DIG-label your targets. Or maybe you are you short on time and need an assay developed quickly? Custom Alpha assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.
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1-25 of 482 Products & Services

  • Tissue Plasminogen Activator (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Tissue Plasminogen Activator (tPA) Detection Kit is designed for detection and quantitation of human tPA in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both tPA and pro-tPA.
    Price Range : 1905.00 USD - 12600.00 USD
  • LDHA (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human lactate dehydrogenase subunit A (LDHA) enables the quantitative determination of human LDHA in cell lysates and serum using a homogeneous AlphaLISA assay (no wash steps). Please note this is NOT a cell cytotoxicity assay, as the assay does not measure secreted LDH.
    Price Range : 912.00 USD - 17600.00 USD
  • Alpha Donor Bead Antibody Conjugated

    Anti-DNP Alpha Donor Beads

    Alpha Donor beads conjugated to anti-DNP antibody. These beads can be used to capture DNP-labeled proteins, peptides, and small molecules.
    Price Range : 1995.00 USD - 23300.00 USD
  • LAG-3 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human LAG-3 enables the quantitative determination of human lymphocyte-activation gene 3 (LAG-3) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 940.00 USD - 18100.00 USD
  • OX40L (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human OX40L enables the quantitative determination of human OX40 ligand in serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 901.00 USD - 13400.00 USD
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    Price Range : 317.00 USD - 12900.00 USD
  • NFL (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human neurofilament light chain protein (NFL) enables the quantitative determination of human FABP3 in buffer and cerebrospinal fluid (CSF) using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 939.00 USD - 21000.00 USD
  • CYR61 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human cysteine rich angiogenic inducer 61 (CYR61) enables the quantitative determination of human Cyr61 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 912.00 USD - 17600.00 USD
  • Alpha Donor Bead Fc-coated

    Fc-fragment (human) conjugated Alpha Donor Beads

    Alpha Donor beads conjugated to human IgG Fc fragment. These beads can be used to capture Fc binding proteins and receptors.
    Price Range : 607.00 USD - 11000.00 USD
  • Streptavidin-coated Alpha Donor Bead
    Price Range : 63.00 USD - 5400.00 USD
  • AlphaLisa Analyte 2

    FABP3 (human), lyophilized (AlphaLISA)

    Recombinant human fatty acid binding protein 3 (FABP3) standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human FABP3 detection kit, but can also be ordered separately.
    List Price : 912.00 USD
  • NNMT (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human Nicotinamide N-Methyltransferase (NNMT) enables the quantitative determination of human NNMT in buffered solution, cell culture media, and serum using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 912.00 USD - 17600.00 USD
  • IL-5 (human) Research kits

    The AlphaLISA® Human Interleukin 5 (IL5) Detection Kit is designed for detection and quantitation of human IL5 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1905.00 USD - 12600.00 USD
  • AlphaLISA Sandwich Anti-AnalyteConjugatedAcceptorBead

    FGF23 (Human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human FGF23 enables the quantitative determination of human FGF23 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The kit demonstrated no cross reactivity to FGF21 and very low reactivity to Mouse FGF23.
    Price Range : 599.00 USD - 12200.00 USD
  • Phospho-SHP-1 (Tyr564) AlphaLISA SureFire Ultra Detection Kit

    The AlphaLISA® SureFire® Ultra™ p-SHP-1 (Tyr564) assay is a sandwich immunoassay for quantitative detection of phospho-SHP-1 (phosphorylated on Tyr564) in cellular lysates using Alpha Technology.
    Price Range : 553.00 USD - 36700.00 USD
  • BTLA (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human BTLA enables the quantitative determination of human B- and T-lymphocyte attenuator (BTLA, CD272) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 940.00 USD - 18100.00 USD
  • FABP3 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human fatty acid binding protein 3 (FABP3) enables the quantitative determination of human FABP3 in buffer, cell cultrue media, and serum using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 901.00 USD - 13300.00 USD
  • Albumin (human) AlphaLISA Biotin-Free Detection Kit

    The AlphaLISA® Human Albumin Biotin-Free Detection Kit is designed for the quantitative detection of human albumin in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
    Price Range : 559.00 USD - 12200.00 USD
  • CCL18 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CCL18 enables the quantitative determination of human chemokine (C-C motif) ligand 18 in buffer, human serum, cell media, and PBMC culture supernatant samples using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 939.00 USD - 13300.00 USD
  • Alpha Terbium SureFire Ultra Multiplex TBSU

    Phospho-STAT1 (Tyr701) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT1 (Tyr701) in a multiplexed assay.
    Price Range : 416.00 USD - 27600.00 USD
  • PCSK9 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Proprotein Convertase Subtilisin / Kexin Type 9 (PCSK9) Detection Kit is designed for detection and quantitation of human PCSK9 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. One antibody targets the pro-domain of PCSK9.
    Price Range : 1905.00 USD - 12600.00 USD
  • Alpha Donor Bead Antibody Conjugated

    Anti-GFP Alpha Donor Beads

    Alpha Donor beads conjugated to anti-GFP antibody. The antibody is a mouse monoclonal antibody that recognizes wildtype and recombinant GFP (rGFP), and cross-reacts with eGFP. These beads can be used to capture GFP-tagged proteins for AlphaScreen, AlphaLISA, and AlphaPlex no-wash assays.
    Price Range : 767.00 USD - 11200.00 USD
  • HLA-C (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human HLA-C enables the quantitative determination of human MHC class I antigen C (HLA-C) in buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 940.00 USD - 18100.00 USD
  • Cry2A AlphaLISA Detection Kit

    The AlphaLISA® Cry2A Detection Kit is designed for detection and quantitation of Cry2A in GMO seeds, leaves, and plant extracts in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 752.00 USD - 11700.00 USD
  • CD40 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CD40 enables the quantitative determination of human cluster of differentiation 40 protein in serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 940.00 USD - 13300.00 USD
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