PerkinElmer

Effects on Bio-organisms

Concern is growing about the impact of microplastics on the marine and freshwater ecosystem, and in particular the effect on the food chain. In addition to leaching harmful additives, microplastics generate a surface biofilm that concentrates organic pollutants such as pesticides and attracts potentially harmful bacteria. This biota is consumed by marine organisms with potentially harmful consequences for the organisms themselves, the food chain, and human health. Issues such as how microplastics affect reproduction, growth, and productivity of bio-organisms, and how changes in immune response can lead to increased susceptibility to infection, are critically important, as they can lead to disruption in the food chain. In terms of physical harm, there is evidence of tiny particles penetrating into tissue, or into the brain, causing behavioral changes. Such research demands cellular imaging and assay technologies, gene sequencing, and proteomic tools.

Typical applications include:

  • Cellular stress and inflammation, oxidative stress, cytotoxicity, cell viability, and hormone analysis
  • Reactive oxygen species (ROS) studies
  • Cell invasion, uptake, and localization studies
  • Localization and analysis of tiny particles embedded within tissue and cells
  • Recognition, characterization, and size calculation of microplastic particles in tissue
  • Imaging for tracking infection and immune response in live fish and crustaceans
  • Next-generation sequencing of biofilm bacterial genomes
  • Nanoparticle analysis
 

For research use only. Not for use in diagnostic procedure.

Solutions

NGS Library Prep Kits

NEXTflex® library preparation kits are designed to reduce bias and increase the sensitivity, flexibility, and speed of library prep for both Illumina® and Ion Torrent sequencing platforms. An extensive selection of NEXTflex single index and dual index barcoded adapters is available to meet your specific multiplexing needs. Automation protocols for the PerkinElmer® Sciclone® NGS Workstation are available for many of the NEXTflex library prep kits for labs requiring higher sample throughput, reduced hands-on time, greater reproducibility, or improved process control.

For research use only. Not for use with diagnostic procedures.

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Opera Phenix High-Content Screening System

The Opera Phenix High Content Screening System is the premier confocal solution for today's most demanding high content applications. Drawing on over a decade of experience with the industry-leading Opera® High Content Screening System, the Opera Phenix is designed for high-throughput, phenotypic screening and assays involving complex disease models, such as live cells, primary cells and microtissues.

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EnSight Multimode Plate Reader

The EnSight multimode plate reader combines labeled & image-based cytometry technologies in a single benchtop platform. Imaging & online data analysis is fast: a 384-well plate can be imaged in less than 5 mins. The system’s modular design, combined with workflow-based Kaleido data acquisition & analysis software, gives a truly versatile plate reader that gets users productive quickly.

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AlphaLISA SureFire Ultra p-JNK1/2/3 (Thr183/Tyr185) HV Assay Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.

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Operetta CLS High-Content Analysis System

Uncover deep biological understanding in your everyday assays and innovative applications using the Operetta CLS high-content analysis system. Featuring a unique combination of technologies, the system delivers all the speed, sensitivity and resolution you need to reveal fine sub-cellular details. And with our simple, powerful Harmony 4.5 software, Operetta CLS lets you find even subtle phenotypic changes.

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Sciclone® G3 NGS and NGSx Workstation

The Sciclone® G3 NGS and NGSx workstations area complete benchtop solution for the automated construction of up to or greater than 96 libraries per day.

Next generation sequencing (NGS) technologies are enabling science at unprecedented rates. As the cost of sequencing declines, throughput continues to increase and the number of applications for which sequencing data can be used is expanding. The Sciclone® G3 NGS and NGSx liquid handling workstation was designed for high throughput, walkaway NGS sample preparation. The deck design and intuitive interface virtually eliminate cross contamination and user error. These liquid handlers simplify the construction of up to or greater than 96 libraries per day.

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Zephyr® G3 NGS Workstation

The Zephyr® G3 NGS workstation is a benchtop liquid handler designed to automate the construction of 48 to 96 next generation sequencing (NGS) libraries per day. The simplified user-interface and integrated hardware maximize laboratory productivity while reducing variability resulting from manual pipetting.

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JANUS® G3 NGS Express Workstation

The JANUS® G3 NGS Express workstation is a benchtop platform designed to simplify and reduce errors in low-to-moderate throughput NGS library construction. It uses an intuitive interface to guide users through protocol selection and deck setup, as well as, displays in-run status and details. An optional enclosure not only helps to minimize sample contamination, but the easy to read status lights provide visual feedback for true walkaway sample preparation.

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