Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.
Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.
Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.
Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.
Ease-of-use: homogeneous, no-wash assay, with no separation steps
Fast: results in 3 hours for most applications
High sensitivity: detect down to femtogram levels of target
Large dynamic range: 4-5 logs
Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more
Choose the Alpha solution that’s right for you
AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
AlphaLISA SureFire® Ultra™ and AlphaScreen®SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.
Homogeneous dispense-and-read technology
384-well and 1536-well formats
LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.
DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.
DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match.
Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
No more washing – just add your lites reagent of choice, mix, incubate, and read
Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.
ATPlite 1step – Highest sensitivity
True “mix and measure” continuous processing
Ultra high sensitivity, 3 times more light output than ATPlite
Short equilibrium time for faster processing
Signal half-life of 30 minutes
ATPlite – Convenient, extended Signal
2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
Human VEGFR 1 (FLT-1) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human FLT-1 Detection Kit, but can also be ordered separately.
The AlphaLISA® Progesterone Detection Kit is designed for detection and quantitation of progesterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
LANCE Ultra phospho-LCK (Tyr394) kits are designed for the detection of phosphorylated LCK in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
LANCE Ultra phospho-Src (Tyr419) kits are designed for the detection of Src (phosphorylated at residue Y419) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
The LANCE®Ultra Human CXCL9/MIG Detection Kit is designed for detection and quantitation of human CXCL9/MIG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The AlphaLISA® immunoassay kit for human MANF enables the quantitative determination of mesencephalic astrocyte-derived neurotrophic factor (MANF) in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT1 (Ser727) in a multiplexed assay.
The AlphaLISA® immunoassay kit for human SCF enables the quantitative determination of stem cell factor (SCF; KIT-ligand) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
The LANCE®Ultra Human IL-5 Detection Kit is designed for detection and quantitation of human interleuukin-5 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The AlphaLISA® immunoassay kit for human interferon lambda-2 (IFN-λ2) enables the quantitative determination of human IFN-λ2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).