PerkinElmer

Drug Discovery Screening Reagents

OF

Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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"Drug Discovery Screening Reagents" 1-50 of 1423 Products and Services

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Factor VIII (human) AlphaLISA Detection Kit

The AlphaLISA® Human Factor VIII Detection Kit is designed for detection and quantitation of Human Factor VIII in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 645.00 USD - 10100.00 USD
AlphaLISA immunoassay kits

NGAL (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for NGAL is designed for the quantitative determination of Neutrophil Gelatinase Associated Lipocalin (NGAL) also known as lipocalin 2 in cell culture media and human serum using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with mouse NGAL, rat NGAL, bovine NGAL and chicken NGAL.
Price Range : 564.00 USD - 10900.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-4E-BP1 (Thr37/46) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-4E-BP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-p70 S6 Kinase (Thr389) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD

IGF-1 (rat) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for rat IGF-1 enables the quantitative determination of rat insulin-like growth factor 1 (IGF-1) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 600.00 USD - 18000.00 USD
PerkinElmer

Alpha SureFire Ultra Lysis Buffer (5X)

Additional buffer for Alpha SureFire Ultra assays.
Price Range : 118.00 USD - 619.00 USD
AlphaLISA immunoassay kits

GDNF (human) AlphaLISA Detection kit

AlphaLISA no-wash immunoassay kit for detection and quantitation of human GDNF (glial cell-derived neurotrophic factor) in serum, plasma, cell culture supernatants, and other samples.
Price Range : 645.00 USD - 10000.00 USD
AlphaLISA immunoassay kits

TNFα (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human TNFα Biotin-Free Detection Kit is designed for the quantitative detection of human TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 695.00 USD - 10100.00 USD
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr421/Ser424) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr421/Ser424) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr421/Ser424) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit
Price Range : 1493.00 USD - 16100.00 USD

LANCE Ultra Europium-anti-phospho-Histone H3 (Thr3) Antibody

Rabbit monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated at Thr3. Histone H3 peptides can be phosphorylated by several kinases including RSK2. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG
Price Range : 1532.00 USD - 7100.00 USD

Nidogen-1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human Nidogen-1 enables the detection and quantitation of Human Nidogen-1 (NID-1; also known as Entactin) in human serum, plasma, and, cell lysyate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 446.00 USD - 9700.00 USD
AlphaLISA SureFire Ultra Lysate

AlphaLISA SureFire Ultra ERK 1/2 pT202/Y204, Lysat

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-ERK1/2 assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-ERK assay kit, but can also be ordered separately.
List Price : 21.00 USD

TNFα (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine TNFα enables the detection and quantitation of porcine TNFα in porcine serum, plasma, and, cell lysyate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimal cross-reactivity with human and bovine TNFα and no cross reactivity towards porcine IFNγ.
Price Range : 622.00 USD - 20200.00 USD
Alpha_Donor_bead_1
Price Range : 56.00 USD - 4776.00 USD
AlphaLISA immunoassay kits

4-1BBL (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human 4-1BBL enables the quantitative determination of 4-1BB ligand in buffer, cell culture media, cell supernatants, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 782.00 USD - 11100.00 USD
AlphaLISA immunoassay kits

IL-21 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-21 enables the quantitative determination of human interleukin-12 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 600.00 USD - 14400.00 USD
PerkinElmer

LANCE Ultra PCSK9, lyophilized (0.3ug)

LANCE Ultra PCSK9, lyophilized (0.3ug)
List Price : 240.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Caspase 9 (Ser196) Kit

The AlphaScreen® SureFire® p-Caspase 9 (Ser196) assay is a sandwich immunoassay for quantitative detection of phospho-Caspase 9 (phosphorylated on Ser196) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLISA immunoassay kits

IL-4 (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine interleukin 4 (IL-4) enables the quantitative determination of porcine IL-4 in cell culture media and serum using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 750.00 USD - 14800.00 USD
AlphaLISA immunoassay kits

Amyloid β 1-42 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for detection and quantitation of human Amyloid β 1-40 (Aβ 1-40) in cerebrospinal fluid (CSF), cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 2912.00 USD - 22800.00 USD
AlphaLisa Analyte 2

Alpha-1 Anti-Trypsin (A1AT) (human), lyophilized (AlphaLISA)

Human A1AT standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human A1AT Detection Kit, but can be ordered separately.
List Price : 240.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT6 (Tyr641) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT6 (Tyr641) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaLISA immunoassay kits

EGFR (human) AlphaLISA Detection Kit

The AlphaLISA® EGFR Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor Receptor (EGFR) in cell culture media, human serum, and other sample types using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 695.00 USD - 10100.00 USD

Fc-fragment (human) conjugated AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to human IgG Fc fragment. These beads can be used to capture Fc binding proteins and receptors.
Price Range : 773.00 USD - 23500.00 USD

p53 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human p53 enables the detection and quantitation of human p53 in serum, buffered solution, or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
List Price : 19600.00 USD

DELFIA® Enhancer, 50mL

DELFIA Enhancer solution for forming fluorescent Terbium and Dysprosium chelates in DELFIA time-resolved fluorescence (TRF) assays.
List Price : 180.00 USD
AlphaLISA SureFire Ultra kits

Phospho-MKK4 (Ser257) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ phospho-MKK4 assay is a sandwich immunoassay for quantitative detection of MKK4 (phosphorylated on Ser257) in cellular lysates using Alpha Technology.

IFN-γ (cynomolgus) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for monkey cynomolgus interferon gamma (IFNγ) enables the quantitative determination of cynomolgus IFNγ in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 433.00 USD - 9500.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-AKT (Thr308) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-AKT (Thr308) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
PerkinElmer

LANCE® Eu-W1024 Anti-phosphotyrosine (PT66)

Europium-labeled anti-phosphotyrosine (PT66) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 1043.00 USD - 9100.00 USD
AlphaLISA immunoassay kits

IL-17A (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-17A enables the detection and quantitation of bovine Interleukin 17A (IL17A) in bovine serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 696.00 USD - 19600.00 USD
SureFire principle

AlphaScreen SureFire p53 (p-Ser15) Assay Kit

The AlphaScreen® SureFire® p-p53 (p-Ser15) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser15) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
PerkinElmer

Alpha SureFire Ultra Activation Buffer

Additional buffer for Alpha SureFire Ultra assays.
Price Range : 118.00 USD - 618.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total GAPDH (Human/Mouse) Assay Kit

The AlphaLISA® SureFire® Ultra Mouse/Human GAPDH assay is a sandwich immunoassay for quantitative detection of human or mouse GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay.
Price Range : 489.00 USD - 28400.00 USD
AlphaLisa Analyte 2

Amyloid β 1-40 (human), lyophilized (AlphaLISA)

Human Amyloid β 1-40, lyophilized, 0.3 µg. Amyloid β 1-40 is included in the AlphaLISA® human Amyloid β 1-40 kits, but can also be ordered separately as a standalone item. One vial contains an amount of human Amyloid β 1-40 sufficient for performing 10 standard curves.
List Price : 247.00 USD
Assay principle for LANCE TR-FRET binding assay

CTLA4-CD80 AlphaLISA Binding Kit

This kit is designed for the detection of binding activity between Human CTLA-4 and CD80, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding to CTLA-4/CD80.
Price Range : 1800.00 USD - 11000.00 USD
LANCE Detection Kit

IL-5 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-5 Detection Kit is designed for detection and quantitation of human interleuukin-5 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 796.00 USD - 10600.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-VEGF Receptor 2 (Tyr1175) Assay Kit

The AlphaLISA® SureFire® Ultrap-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
LANCE Detection Kit

Tau (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human TAU Detection Kit is designed for detection and quantitation of human Tau in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 796.00 USD - 10600.00 USD

IL-1β (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IL1β in bovine serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 645.00 USD - 10300.00 USD
SureFire principle

AlphaScreen SureFire Akt 1/2 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt 1/2 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt 1/2 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLisa Analyte 2

TIMP-1 (human), lyophilized (AlphaLISA)

Human tissue inhibitor of metalloproteinases-1 (TIMP-1) standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human TIMP-1 detection kit, but can also be ordered separately.
List Price : 516.00 USD

D-dimer (human) AlphaLISA Detection Kit

The AlphaLISA® Human D-dimer Detection Kit is designed for detection and quantitation of human D-dimer in citrate plasma, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Please note the kit shows some cross-reactivity to D-monomer.
Price Range : 2912.00 USD - 22800.00 USD
AlphaLISA immunoassay kits

p24 (human HIV) AlphaLISA Detection Kit

The AlphaLISA® Human Immunodeficiency Virus type-1 p24 protein (HIV p24) Detection Kit is designed for detection and quantitation of HIV p24 in buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1520.00 USD - 10100.00 USD
AlphaLISA immunoassay kits

EGF/EGFR AlphaLISA Binding Kit

The AlphaLISA® EGF/EGFR Binding Kit is designed for the detection of binding activity between Human EGF and EGFR, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of therapeutics which competitively inhibit the EGF/EGFR interaction.
Price Range : 695.00 USD - 9700.00 USD
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr229) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr229) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr229) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
SureFire principle

AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLisa Analyte 2

IL-22 (human), lyophilized (AlphaLISA)

Human IL-22 standard, 1 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA human/rat IL-22 detection kits, but can also be ordered separately.
List Price : 247.00 USD
SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit 

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD

AlphaScreen PhosphoSensor Kit

Antibody-free assay for kinase activity via dectection of phosphorylated protein or peptide substrate.
Price Range : 384.00 USD - 2878.00 USD

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