PerkinElmer

Reagents for Biomarker Detection

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Our biomarker detection reagents and kits are designed to excel in applications that rely on high sensitivity, wide dynamic range, and easy automation. With no wash or separation steps necessary, our homogeneous AlphaLISA® immunoassays achieve faster run-times than any ELISA assay on the market.

Our portfolio of reagents for biomarker detection include:

  • AlphaLISA® immunoassay kits for high sensitivity and large signal to background in a variety of sample matrices, from human serum to cell culture media to CSF
  • LANCE® TR-FRET analyte detection technology
  • DELFIA (dissociation-enhanced lanthanide fluorescence immunoassay) TRF ELISA alternative technology

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  • Application Note

    Imaging Hepatocellular Liver Injury using NIR-labeled Annexin V

    Drug induced liver injury (DILI) is a major reason for late stage termination of drug discovery research projects, highlighting the importance of early integration of liver safety assessment in the drug development process. A technical approach for in vivo toxicology determination was developed using Acetaminophen (APAP), a commonly used over-the-counter analgesic and antipyretic drug, to induce acute hepatocellular liver injury.

  • Application Note

    Multiplex 2D Imaging of NIR Molecular Imaging Agents on the IVIS SpectrumCT and FMT 4000

    Epifluorescence (2D) imaging of superficially implanted mouse tumor xenograft models offers a fast and simple method for assessing tumor progression or response to therapy. This approach for tumor assessment requires the use of near infrared (NIR) imaging agents specific for different aspects of tumor biology, and this Application Note highlights the ease and utility of multiplex NIR fluorescence imaging to characterize the complex biology within tumors growing in a living mouse.

  • Poster

    Combined efficacy & toxicity imaging following acute 5-FU treatment of HT-29 tumor xenografts

    Cancer chemotherapy can produce severe side effects such as suppression of immune function and damage to heart muscle, gastrointestinal tract, and liver. If serious enough, tissue injury can be a major reason for late stage termination of drug discovery research projects, so it is becoming more important to integrate safety/toxicology assessments earlier in the drug development process. There are a variety of traditional serum markers, tailored mechanistically to specific tissues, however there are no current non-invasive assessment tools that are capable of looking broadly at in situ biological changes in target and non-target tissue induced by chemical insult.