PerkinElmer

AlphaPlex Multiplexing Kits

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AlphaPlex is a homogeneous, all-in-one-well multiplexing reagent technology that provides highly sensitive detection of a wide range of analytes in a simple no-wash assay format based on our proven Alpha Technology. Now you can quantify multiple analytes in a single well for faster and more accurate results, even with low sample volumes.

  • High sensitivity and accuracy
  • High well-to-well reproducibility
  • Wide range of analytes: from small nucleotides and large (MDa) proteins to scarce biological samples (primary and stem cells)
  • Various affinities: from pM to mM (e.g. lectin-sugar interaction)
  • Cost-savings from a 5-20 times reduction in sample consumption compared to other multiplexing platforms
  • Simple, no-wash assay for easier set-up, saving you time and effort
  • Use with Alpha-enabled filter-based readers such as the EnVision® Multilabel Plate Reader, and JANUS® Automated Workstation

The AlphaPlex assay technology enables easy, accurate multiplex quantification of a wide range of analytes, from large proteins to small proteins and scarce biological samples such as primary cells and stem cells, for increased predictivity and confidence in your results. It can be applied to a wide range of application including:

  • Biomarkers for PD/PK
  • Biomarkers for stem cells
  • Kinase: total vs phosphorylated protein
  • Epigenetic markers: total histone vs specific marker
  • Amyloid peptides
  • IgG profiling / testing
  • Assay normalization with housekeeping proteins

AlphaPlex is a homogeneous luminescent proximity reagent technology that allows you to quantify multiple analytes in a single well. When the Donor and Acceptor beads are brought together, a cascade of chemical reactions is set in motion, causing a greatly amplified signal. By using multiple Acceptor beads which emit different wavelengths, multiple analytes can be detected.

Products & Services (19)
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Assay Target
  • ICAM-1 (2)
  • IFNγ (2)
  • IL17 (1)
  • IL17A (1)
  • IL1β (2)
  • IL2 (2)
  • IL22 (1)
  • IL6 (1)
  • Insulin (2)
  • IP10 (2)
  • TNFα (2)
  • α-tubulin (1)
Application
  • Multiplexing (9)
  • Protein Analysis & Detection (1)
Therapeutic Area
  • Cardiovascular (2)
  • Inflammation (14)
  • Metabolic (2)
Assay points(200-50,000)
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  • 5000 (4)
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  • Cytokine (14)
  • Hormone (2)
  • Protein (3)
Experimental Type
  • In vitro (12)
Format
  • Microplates (18)

1-12 of 19 Products & Services

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  • AlphaPlex Terbium immunoassay kits

    AlphaPlex-545 Human IL17 Detection Kit

    The AlphaPlex™ 545 (Terbium) Human IL17 Detection Kit is designed for the quantitative determination of human IL17 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
    Price Range : 374.00 USD - 10500.00 USD
  • AlphaPlex Terbium immunoassay kits

    AlphaPlex-545 (Tb) Human IP10 Detection Kit

    The AlphaPlex Terbium (Tb) Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.
    Price Range : 717.00 USD - 10500.00 USD
  • AlphaPlex Samarium immunoassay kits

    AlphaPlex-645 (Sm) Human IFN-γ Detection Kit

    The AlphaPlex™ 645 (Samarium) Human Interferon-gamma (IFN-γ) Detection Kit is designed for the quantitative determination of human IFN-γ together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
    Price Range : 480.00 USD - 10500.00 USD
  • AlphaPlex Terbium immunoassay kits

    AlphaPlex-545 Human/Rat IL22 Detection kit

    The AlphaPlex 545 (Terbium) Human / Rat IL22 Detection Kit is designed for the quantitative determination of human or rat IL22 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
    Price Range : 672.00 USD - 10500.00 USD
  • AlphaPlex Samarium immunoassay kits

    AlphaPlex-645 (Sm) Human TNFα Detection Kit

    The AlphaPlex™ 645 (Samarium) Human TNFα Detection Kit is designed for the quantitative determination of human TNFα together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
    Price Range : 633.00 USD - 10500.00 USD
  • AlphaPlex Terbium immunoassay kits

    AlphaPlex-545 (Tb) Human IL2 Detection Kit

    The AlphaPlex™ 545 (Terbium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
    Price Range : 633.00 USD - 10500.00 USD
  • AlphaPlex Terbium immunoassay kits

    α-tubulin (human) AlphaPlex Terbium Detection Kit

    The AlphaPlex™ Terbium (Tb) Human α-Tubulin Detection Kit is designed for the quantitative determination of human α-tubulin in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay. This assay can be used as a cell normalization or protein level normalization assay, in conjunction with AlphaLISA® biomarker detection kits.
    Price Range : 759.00 USD - 14600.00 USD
  • AlphaPlex Terbium immunoassay kits

    AlphaPlex-545 (Tb) Human TNFα Detection Kit

    The AlphaPlex™ 545 (Terbium) Human TNFα Detection Kit is designed for the quantitative determination of human TNFα together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
    Price Range : 633.00 USD - 10500.00 USD
  • AlphaPlex Samarium immunoassay kits

    AlphaPlex-645 (Sm) Human IL1β Detection Kit

    The AlphaPlex™ 645 (Samarium) Human IL1β Detection Kit is designed for the quantitative determination of human insulin together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
    Price Range : 613.00 USD - 10500.00 USD
  • AlphaPlex Terbium immunoassay kits

    AlphaPlex-545 (Tb) Human ICAM-1 Detection Kit

    The AlphaPlex™-545 (Tb) Human ICAM-1 Detection Kit is designed for detection and quantitation of human ICAM-1 in cell culture supernatant and human serum using a homogeneous AlphaPlex multiplexing assays (no wash steps).
    Price Range : 672.00 USD - 23700.00 USD
  • AlphaPlex Terbium immunoassay kits

    AlphaPlex-545 (Tb) Human IFN-γ Detection Kit

    The AlphaPlex™ 545 (Terbium) human interferon-gamma (IFN-γ) Detection Kit is designed for the quantitative determination of human IFN-γ together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
    Price Range : 480.00 USD - 10500.00 USD
  • AlphaPlex Terbium immunoassay kits

    AlphaPlex-545 Human IL1β Detection Kit

    The AlphaPlex™ 545 Human IL1β Detection Kit is designed for the quantitative determination of human IL1β together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
    Price Range : 613.00 USD - 10500.00 USD
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