PerkinElmer

AlphaLISA Binding Kits

OF

The interactions and binding of proteins are implicated in a large number of biological processes. The need for an efficient, highly sensitive assay to study large protein interactions is increasingly important. Alpha Technology is a highly flexible, homogeneous, no-wash assay ideal for the measurement of protein interactions and complexes as large as 200 nm in size. A bead-based proximity assay, Alpha Technology offers the possibility to assay many biological targets, including enzymes, receptor-ligand interactions, low affinity interactions, second messenger levels, DNA, RNA, proteins, peptides, sugars and small molecules.

Features:

  • No-wash steps, no separation steps
  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

Products & Services (20)
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Filters :

Assay Target
  • 4-1BB (1)
  • 4-1BBL (1)
  • CD80 (1)
  • CD86 (1)
  • CTLA4 (2)
  • EGF (1)
  • EGFR (1)
  • FCGR1 (1)
  • FCGR2A (2)
  • FCGR2B (1)
  • FCGR3A (2)
  • HLA-DRA (1)
  • LAG3 (1)
  • LDLR (1)
  • PCSK9 (1)
  • PD-1 (2)
  • PD-L1 (2)
  • PDGF-BB (2)
  • PDGFR (2)
  • TNFα (2)
  • TNRFR1 (1)
  • TNRFR3 (1)
Target Species
  • Human (13)
  • Mouse (1)
Application
  • Protein Analysis & Detection (8)
  • Protein-protein interactions (19)
Therapeutic Area
  • Biologics/Bioprocess (6)
  • Cancer (9)
  • Cardiovascular (1)
  • Inflammation (2)
Assay Points (200-50,000)
  • 100 (4)
  • 500 (20)
  • 5000 (20)
Assay Target Class
  • Cytokine (2)
  • Fc-receptor (6)
  • Protein (12)
Experimental Type
  • In vitro (4)
Format
  • Microplates (4)

1-12 of 20 Products & Services

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  • Assay principle for AlphaLISA TNFa/TNFR1 binding kit

    TNFα/TNFR1 (human) AlphaLISA Binding Kit

    The AlphaLISA® human TNFα/TNFR1 binding kit is designed for the detection of binding activity between human TNFα and TNFR1, using a fast and simple homogeneous AlphaLISA assay (no wash steps). This assay can be used to screen for small molecules that inhibit binding, as a competitive ligand binding (CLB) assay to screen therapeutic blocking antibodies, and for potency assays.
    Price Range : 2213.00 USD - 11400.00 USD
  • FcRn (human) AlphaLISA Binding Kit

    The AlphaLISA human FcRn binding kit is designed for the detection of binding between FcRn and human IgG using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics using a competition assay format.
    Price Range : 2213.00 USD - 11100.00 USD
  • AlphaLISA binding assay

    PDGF-BB/PDGFRβ (human) AlphaLISA Binding Kit

    This kit is designed for the detection of binding activity between Human PDGF-BB and PDGFRβ, using a homogeneous AlphaLISA assay (no wash step). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to PDGF-BB and PDGFRβ.
    Price Range : 1821.00 USD - 14600.00 USD
  • Assay principle for LANCE TR-FRET binding assay

    CTLA4-CD86 AlphaLISA Binding Kit

    The AlphaLISA® CTLA-4/CD86 binding kit is designed for the detection of binding activity between human CTLA-4 and CD86, using a fast and simple homogeneous AlphaLISA assay (no wash steps). This assay can be used to screen for small molecules that inhibit binding, as a competitive ligand binding (CLB) assay to screen therapeutic blocking antibodies, and for potency assays.
    Price Range : 1876.00 USD - 11400.00 USD
  • AlphaLISA immunoassay kits

    FCGR1/CD64 AlphaLISA Binding Kit

    This kit is designed for the detection of binding between FCGR1 (CD64) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
    Price Range : 2213.00 USD - 11100.00 USD
  • FcγR3B (CD16b) AlphaLISA Binding Kit

    The AlphaLISA FCGR3B (CD16b) binding kit is designed for the detection of binding between FcγR3B (CD16b) and human IgG Fc fragment using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics using a competition assay format.
    Price Range : 2213.00 USD - 11100.00 USD
  • Assay principle for LANCE TR-FRET binding assay

    CTLA4-CD80 AlphaLISA Binding Kit

    The AlphaLISA® CTLA-4/CD80 binding kit is designed for the detection of binding activity between human CTLA-4 and CD80, using a fast and simple homogeneous AlphaLISA assay (no wash steps). This assay can be used to screen for small molecules that inhibit binding, as a competitive ligand binding (CLB) assay to screen therapeutic blocking antibodies, and for potency assays.
    Price Range : 1876.00 USD - 11400.00 USD
  • Assay principle for AlphaLISA mouse PD-1/PD-L1 binding assay

    PD-1/PD-L1 (mouse) AlphaLISA Binding Kit

    The AlphaLISA® mouse PD-1/PD-L1 binding assay enables development and characterization of small and large molecule anti-mouse PD-1 or PD-L1 reagents for in vitro and in vivo studies, testing of other putative binding ligands such as PD-L2 or characterization of ligand subregions for their ability to block binding, and determination of mouse cross-reactivity of anti-human PD-1 or PD-L1 reagents using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 2213.00 USD - 11100.00 USD
  • AlphaLISA binding assay

    PDGF-BB/PDGFRα (human) AlphaLISA Binding Kit

    This kit is designed for the detection of binding activity between Human PDGF-BB and PDGFRα, using a homogeneous AlphaLISA assay (no wash step). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to PDGF-BB and PDGFRα.
    Price Range : 1821.00 USD - 14600.00 USD
  • Assay principle for AlphaLISA TNFa/TNFR2 binding kit

    TNFα/TNFR2 (human) AlphaLISA Binding Kit

    The AlphaLISA® human TNFα/TNFR2 binding kit is designed for the detection of binding activity between human TNFα and TNFR2, using a fast and simple homogeneous AlphaLISA assay (no wash steps). This assay can be used to screen for small molecules that inhibit binding, as a competitive ligand binding (CLB) assay to screen therapeutic blocking antibodies, and for potency assays.
    Price Range : 2213.00 USD - 11400.00 USD
  • FcγR2A/CD32a (167H) AlphaLISA Binding Kit

    This kit is designed for the detection of binding between FCGR2A (CD32a) (167H) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics by using competitive binding.
    Price Range : 2213.00 USD - 11100.00 USD
  • Assay principle for AlphaLISA binding assay

    4-1BB/4-1BBL (human) AlphaLISA Binding Kit

    The AlphaLISA® 4-1BB/4-1BBL binding kit is designed for the detection of binding activity between human 4-1BB and 4-1BBL, using a fast and simple homogeneous AlphaLISA assay (no wash steps). This assay can be used to screen for small molecules that inhibit binding, as a competitive ligand binding (CLB) assay to screen therapeutic blocking antibodies, and for potency assays.
    Price Range : 2229.00 USD - 11100.00 USD
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