Alpha SureFire® Technology is a reagent platform for rapid, sensitive, and quantitative detection of protein phosphorylation from cells. It is based on the proven Alpha Technology, a very versatile platform that eliminates time-consuming separation and wash-steps. The SureFire sandwich bead-based immunoassays bring Alpha Donor and Acceptor beads together, causing a cascade of chemical reactions to create a greatly amplified signal to measure endogenous protein phosphorylation. It can also be used with transfected cells and enables the precise interrogation of various signalling pathways, receptors and kinase targets in a cell-based format.
All the Alpha SureFire kits provide:
The Alpha SureFire® Ultra™ Multiplex Phospho + Total assay kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of the total amount of the same protein. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm).
The two distinct Alpha Acceptor beads report their binding to a distinct antigen through their association with specific assay antibodies, as indicated below.
The next-generation technology for detecting protein phosphorylation, AlphaLISA SureFire Ultra is a highly sensitive assay for screening therapeutic antibodies and small molecules on cultured cells or tissue lysates. AlphaLISA SureFire Ultra combines CaptSure™ technology with AlphaLISA Acceptor beads for a more efficient, faster, and simpler assay protocol.
With CaptSure™ technology, there is no interference from spiked antibodies, and with a lower background signal, you have better sensitivity and dynamic range, resulting in more – and more accurate – information from all your samples.
The traditional platform for a wide variety of applications, AlphaScreen® SureFire® is a no-wash assay for the detection of phosphorylated proteins in cells. The AlphaScreen SureFire kits use an immunosandwich assay based on a combination of protein A and streptavidin for the capture of the sandwich antibodies.
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