PerkinElmer

Alpha SureFire Kits

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Alpha SureFire® Technology is a reagent platform for rapid, sensitive, and quantitative detection of protein phosphorylation from cells. It is based on the proven Alpha Technology, a very versatile platform that eliminates time-consuming separation and wash-steps. The SureFire sandwich bead-based immunoassays bring Alpha Donor and Acceptor beads together, causing a cascade of chemical reactions to create a greatly amplified signal to measure endogenous protein phosphorylation. It can also be used with transfected cells and enables the precise interrogation of various signalling pathways, receptors and kinase targets in a cell-based format.

All the Alpha SureFire kits provide:

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal

Alpha SureFire Multiplex assay principle
Alpha SureFire Multiplex assay principle

Alpha SureFire® Ultra Multiplex

The Alpha SureFire® Ultra™ Multiplex assay kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of the total amount of the AKT, ERK or cofilin protein or combined with the measurement of a second phosphoprotein. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm). The two distinct Alpha Acceptor beads report their binding to a distinct antigen through their association with specific assay antibodies, as indicated below.

Alpha SureFire Ultra Multiplex assays are divided into two categories (1) Multiplex SureFire Ultra (MPSU) kits and (2) Terbium SureFire Ultra (TBSU) kits. The MPSU kits are complete kits for phospho and total detection of the same target (ERK or AKT), while the TBSU kits measure a single phosphoprotein target and are intended to be combined with a standard AlphaLISA SureFire Ultra kit (ALSU) to assess the second target. The TBSU kits allow for a pick-and-pack combination of any two phosphoprotein targets in the assay lists, providing measurement of two separate targets with ultimate flexibility of selection.

  • High sensitivity and dynamic range – detect low levels of protein phosphorylation, with internal normalization for total protein content
  • No interference from spiked antibodies – uses CaptSure™ technology instead of protein A
  • 2- incubation protocol that can be run in 2 hrs for faster results
  • Convenience of choosing HTS-compatible version or 96-well High Volume (HV) format for low-throughput applications - for all the targets
  • More than 1000 combinations of either phospho X phospho target or phospho x total targets are available
AlphaLISA SureFire assay principle
AlphaLISA SureFire assay principle

AlphaLISA® SureFire® Ultra

The next-generation technology for detecting protein phosphorylation, AlphaLISA SureFire Ultra is a highly sensitive assay for screening therapeutic antibodies and small molecules on cultured cells or tissue lysates. AlphaLISA SureFire Ultra combines CaptSure™ technology with AlphaLISA Acceptor beads for a more efficient, faster, and simpler assay protocol.

  • High sensitivity and dynamic range – detect low levels of protein phosphorylation
  • No interference from spiked antibodies – uses CaptSure™ technology instead of protein A
  • Almost 2-fold increase in 384-well plates working volumes for easier pipetting, compared to AlphaScreen SureFire
  • 1- or 2- incubation protocol that can be run in 2 hrs for faster results
  • Convenience of choosing HTS-compatible version or 96-well High Volume (HV) format for low-throughput applications - for all the targets
  • Ideal tool when working with samples such as tissue lysates - AlphaLISA Acceptor beads have better spectral characteristics with regard to hemoglobin light quenching

With CaptSure™ technology, there is no interference from spiked antibodies, and with a lower background signal, you have better sensitivity and dynamic range, resulting in more – and more accurate – information from all your samples.

AlphaScreen SureFire assay principle
AlphaScreen SureFire assay principle

AlphaScreen® SureFire®

 

The traditional platform for a wide variety of applications, AlphaScreen® SureFire® is a no-wash assay for the detection of phosphorylated proteins in cells. The AlphaScreen SureFire kits use an immunosandwich assay based on a combination of protein A and streptavidin for the capture of the sandwich antibodies.

  • Simple protocol that can be run in 4 hrs
  • Great sensitivity and dynamic range
  • Convenience of choosing HTS-compatible version or 96-well High Volume (HV) format for low-throughput applications - for some targets only
  • Currently the widest choice of phospho-targets in our catalogue
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"Alpha SureFire Kits" 1-50 of 153 Products and Services

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Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-Ribosomal Protein S6 (Ser240/244) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-Ribosomal Protein S6 (Ser240/244) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
SureFire principle

AlphaScreen SureFire MKK3 (p-Ser189), MKK6 (p-Ser207) Assay Kit

The AlphaScreen® SureFire® p-MKK3 (Ser207) assay is a sandwich immunoassay for quantitative detection of phospho-MKK3 (phosphorylated on Ser207) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLISA SureFire Ultra kits

Phospho-Cofilin (Ser3) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 29500.00 USD
SureFire principle

AlphaScreen SureFire IKKb (p-Ser177/Ser181) Assay Kit

The AlphaScreen® SureFire® p-IKKα (Ser177/181) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser177/181) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-p70 S6K (Thr389) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-p70 S6K (Thr389) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-AKT1/2/3 (Thr308) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
SureFire principle

AlphaScreen SureFire Phospho-p38 MAPK Kit

The AlphaScreen® SureFire® p-38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT4 (Tyr693) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT4 (Tyr693) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-IKKα (Ser176/180) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-IKKα (Ser176/180) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
alphascreen surefire total

AlphaScreen SureFire Akt 1/2/3 Total Assay Kit

The AlphaScreen® SureFire® Akt 1/2/3 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1/2/3 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr421/Ser424) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr421/Ser424) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr421/Ser424) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit
Price Range : 1493.00 USD - 16100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SYK (Tyr525/526) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SYK (Tyr525/526) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT5 (Tyr694/699) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT5 (Tyr694/699) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-EGF Receptor (Tyr1068) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-EGF Receptor (Tyr1068) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SMAD1 (Ser463/465) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SMAD1 (Ser463/465) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
SureFire principle

AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
alphascreen surefire total

AlphaScreen SureFire Total Akt1 research kit

The AlphaScreen® SureFire® Akt 1 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD
AlphaLISA SureFire Ultra kits

Phospho-c-Met (Tyr1234/1235) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 29500.00 USD
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr37/Thr46) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT1 (Ser727) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT1 (Ser727) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ HV Multiplex p-ERK 1/2 (Eu) + Total ERK 545 (Tb) Kit

The Alpha SureFire® Ultra™ HV Multiplex p-ERK 1/2 + Total ERK assay kit is used to measure both the phosphorylation (Thr202/Tyr204) and total levels of endogenous “extracellular signal-regulated kinase 1 and 2” (ERK 1/2) in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure ERK 1/2 activation by either recombinant or endogenous receptors, and can be applied to primary cells.
Price Range : 880.00 USD - 51100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-CREB (Ser133) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-CREB (Ser133) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-VEGF Receptor 2 (Tyr1175) Assay Kit

The AlphaLISA® SureFire® Ultrap-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-p70 S6 Kinase (Thr389) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit

The AlphaLISA® SureFire® Ultra p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit 

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-GSK-3β (Ser9) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-GSK-3β (Ser9) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
SureFire principle

AlphaScreen SureFire IKKa (p-Ser176/Ser180) Assay Kit

The AlphaScreen® SureFire® p-IKKα (Ser176/180) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser176/180) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD
SureFire principle

AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (S473) (Eu) + Total Akt1 (Tb) Kit

The Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (Ser473) + Total Akt 1 assay kit is used to measure both the phosphorylation (Ser473) and total levels of endogenous Akt in cellular lysates. The kit measures phosphorylation on Ser473 of Akt 1/2/3, and total levels of Akt 1 only. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt (Ser473) activation by either recombinant or endogenous receptors, and can be applied to primary cells.
Price Range : 880.00 USD - 51100.00 USD
alphascreen surefire total

Total ERK (monoclonal) AlphaScreen SureFire Assay Kit

The AlphaScreen® SureFire® total ERK assay is a sandwich immunoassay for quantitative detection of ERK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

The AlphaLISA® SureFire® Ultra p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT1 (Tyr701) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT1 (Tyr701) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SLP-76 (Ser376) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SLP-76 (Ser376) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT5 (Tyr694/699) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. This kit replaces ALSU-PST5-A-HV. ALSU-PST5-A-HV is no longer available as the antibody(ies) have been discontinued by the supplier. A new antibody pair has been selected and validated to make this new version, with a similar or slightly improved performance over the initial version. Comparative data are included in the manuals of the new kits.
Price Range : 489.00 USD - 28400.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-VEGF Receptor 2 (Tyr1175) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-VEGF Receptor 2 (Tyr1175) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SMAD3 (Ser423/425) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SMAD3 (Ser423/425) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
SureFire principle

AlphaScreen SureFire Akt 1 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-eIF2α (Ser51) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-eIF2α (Ser51) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr70) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr70) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr70) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLISA SureFire Ultra kits

Phospho-p38 MAPK (Thr180/Tyr182) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Tyr180/182) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 29500.00 USD
AlphaLISA SureFire Ultra kits

Phospho-TrkA (Tyr674/675)/phospho-TrkB (Tyr706/707) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-TrkA (Tyr674/675)/p-Trk-B (Tyr706/707) assay is a sandwich immunoassay for quantitative detection of phospho-TrkA (phosphorylated on Tyr674/675) and/or pTrkB (phosphorylated on residues Tyr706/707) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 29500.00 USD
AlphaLISA SureFire Ultra kits

Phospho-Ribosomal Protein S6 (Ser240/244) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.
Price Range : 489.00 USD - 28400.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Insulin Receptor β (Tyr1150/1151) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-Insulin Receptor β (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-INSR (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
SureFire principle

AlphaScreen SureFire CHK1 (p-Ser345) Assay Kit

The AlphaScreen® SureFire® p-CHK1 (Ser345) assay is a sandwich immunoassay for quantitative detection of phospho-CHK1 (phosphorylated on Ser345 in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
SureFire principle

AlphaScreen SureFire ALK (p-Tyr1604) Assay Kit

The AlphaScreen® SureFire® p-ALK (Tyr1604) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1604) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
SureFire principle

AlphaScreen SureFire Phospho-GSK 3b (Ser9) Kit

The AlphaScreen® SureFire® p-GSK 3β (Ser9) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3β (phosphorylated on Ser9) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD
SureFire principle

AlphaScreen SureFire STAT1 (p-Tyr701) Assay Kit

The AlphaScreen® SureFire® p-STAT1 (p-Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-4E-BP1 (Thr37/46) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-4E-BP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
SureFire principle

AlphaScreen SureFire S6RP (p-Ser235;Ser236) Assay Kit

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser235/236) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser235/236) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately, in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD

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