Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.
Ease-of-use: homogeneous assay with no wash requirement and no separation steps
Fast: results in 3 hours for most applications
High sensitivity: detect down to femtogram levels of target
Large dynamic range: 4-5 logs
Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more
Choose the homogenous assay right for you:
AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
AlphaLISA®SureFire®Ultra and AlphaScreen®SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own
Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.
All-in-one-well alternative to Western Blotting
Flexibility to use cultured cell lines, stem cells and primary cells
Cost-effective and flexible – a single plate can accommodate any assay for any target
Great sensitivity and dynamic range
No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Handle large biomolecular interactions with ease.
Ease-of-use: few addition steps, fast assay development
Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
Conserve reagents: robust assays using nM concentrations of proteins/antibodies
Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.
Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
Human VEGFR 1 (FLT-1) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human FLT-1 Detection Kit, but can also be ordered separately.
The AlphaLISA® Progesterone Detection Kit is designed for detection and quantitation of progesterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
The AlphaLISA® immunoassay kit for human MANF enables the quantitative determination of mesencephalic astrocyte-derived neurotrophic factor (MANF) in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT1 (Ser727) in a multiplexed assay.
The AlphaLISA® immunoassay kit for human SCF enables the quantitative determination of stem cell factor (SCF; KIT-ligand) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
The AlphaLISA® immunoassay kit for human interferon lambda-2 (IFN-λ2) enables the quantitative determination of human IFN-λ2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Unconjugated AlphaScreen® Acceptor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own AlphaScreen Acceptor beads for use in Alpha no-wash assays.
The AlphaLISA® Human Insulin Detection Kit is designed for detection and quantitation of human insulin in cell culture supernatants, serum, and plasma using a homogeneous (no-wash steps, no separation steps) assay.
The AlphaScreen®SureFire® p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.