PerkinElmer

Alpha Reagents

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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1-12 of 1040 Products & Services

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  • AlphaLisa Analyte 2

    LDHA (human), liquid (AlphaLISA)

    Human lactate dehydrogenase A subunit recombinant protein standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human LDHA Detection Kit, but can also be ordered separately.
    List Price : 409.00 USD
  • AlphaPlex-545-antibody-bead2

    Anti-FLAG AlphaPlex-545 (Tb) Acceptor beads

    AlphaPlex-545 (Terbium) Acceptor beads conjugated to anti-FLAG antibody, for use in Alpha no-wash multiplexing assays.
    Price Range : 612.00 USD - 25100.00 USD
  • IRAK4 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human interleukin 1 receptor-associated kinase 4 (IRAK4) enables the quantitative determination of human IRAK4 in buffer, lysates, and tissue homogenates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 750.00 USD - 11100.00 USD
  • AlphaLISA immunoassay kits

    Beta-2 Microglobulin (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human beta-2 microglobulin enables the quantitative determination of human beta-2 microglobulin in buffered solution, serum, CSF, urine, saliva, cell culture supernatants, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1821.00 USD - 14600.00 USD
  • AlphaLISA anti-mouse IgG Acceptor beads

    AlphaLISA Acceptor beads conjugated to anti-mouse IgG antibody. This antibody is not Fc-specific; for Fc-specific beads, please see product AL105.
    Price Range : 589.00 USD - 24200.00 USD
  • AlphaPlex Samarium Acceptor Beads

    Anti-FLAG AlphaPlex-645 Acceptor Beads

    AlphaPlex-645 (Samarium) Acceptor beads conjugated to anti-FLAG antibody, for use in Alpha no-wash multiplexing assays.
    Price Range : 612.00 USD - 25100.00 USD
  • AlphaLISA SureFire Ultra kits

    Total Cofilin AlphaLISA SureFire Ultra Detection Kit

    The AlphaLISA® SureFire® Ultra Total Cofilin assay is a sandwich immunoassay for quantitative detection of cofilin (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
    Price Range : 504.00 USD - 29200.00 USD
  • IL-2 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Interleukin-2 (IL2) Detection Kit is designed for detection and quantitation of human IL-2 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1585.00 USD - 10500.00 USD
  • AlphaLISA immunoassay kits

    pNFH (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human phosphorylated neurofilament heavy chain protein (pNFH) enables the quantitative determination of human pNFH in plasma or cerebrospinal fluid (CSF) using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 750.00 USD - 11100.00 USD
  • AlphaLisa Analyte 2

    KIM-1 (human), lyophilized (AlphaLISA)

    Human KIM-1 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human KIM-1 Detection Kit, but can also be ordered separately.
    List Price : 409.00 USD
  • SureFire principle

    AlphaScreen SureFire IkBalpha (p-Ser32/Ser36) Assay Kit

    The AlphaScreen® SureFire® p-IκBα (Ser32/36) assay is a sandwich immunoassay for quantitative detection of phospho-IκBα (phosphorylated on Ser32/36) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1538.00 USD - 16600.00 USD
  • AlphaLISA binding assay

    PDGF-BB/PDGFRβ (human) AlphaLISA Binding Kit

    This kit is designed for the detection of binding activity between Human PDGF-BB and PDGFRβ, using a homogeneous AlphaLISA assay (no wash step). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to PDGF-BB and PDGFRβ.
    Price Range : 1821.00 USD - 14600.00 USD
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