PerkinElmer

Alpha Reagents

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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AlphaLISA immunoassay kits

ApoB (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ApoB enables the quantitative determination of human apolipoprotein B in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 625.00 USD - 18800.00 USD

PER.C6® HCP AlphaLISA Detection Kit

The AlphaLISA® PER.C6® HCP Detection Kit is designed for the quantitative detection of PER.C6® contaminants in buffer and cell culture media using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 1539.00 USD - 10200.00 USD
AlphaLisa Analyte 2

Aβ 1-40 (human), lyophilized (AlphaLISA)

Human Aβ 1-40 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human Aβ 1-40 Detection Kit, but can be ordered separately.
List Price : 250.00 USD
AlphaLISA immunoassay kits

PSMA (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human PSMA enables the quantitative determination of human prostate specific membrane antigen (PSMA) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 625.00 USD - 15000.00 USD
AlphaLISA immunoassay kits

IL-17F (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for IL17F is designed for the quantitative determination of human IL17F in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human IL-17A and with mouse IL17F, bovine IL17F, and rat IL17F. Cross-reactivity with other species has not tested.
Price Range : 644.00 USD - 19300.00 USD
AlphaLisa Analyte 2

CXCL10/IP-10 (human), lyophilized (AlphaLISA)

Human C-X-C Motif Chemokine 10 / 10 kDa Interferon- gamma-Induced Protein (CXCL10 / IP-10) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human CXCL10/IP-10 kit, but can be ordered separately.
List Price : 257.00 USD

Heme oxygenase 2 AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human heme oxygenase 2 (HO-2) enables the quantitative determination of human HO-2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 451.00 USD - 9900.00 USD
AlphaLisa Analyte 2

CCL2/MCP-1 (mouse), lyophilized (AlphaLISA)

Mouse C-C Motif Chemokine 2 / Monocyte Chemoattractant Protein 1 (CCL2/MCP1) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse/Rat CCL2/MCP1 kit, but can be ordered separately.
List Price : 552.00 USD
AlphaLISA immunoassay kits

Laminin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human laminin enables the detection and quantitation of human laminin in human serum, plasma, lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human IL-28A, IL-29, and Mouse Laminin.
Price Range : 465.00 USD - 10200.00 USD
AlphaPlex Samarium Acceptor Beads

Anti-Mouse IgG AlphaPlex™ 645 (Sm) Acceptor Beads

AlphaPlex™-645 (Samarium) Acceptor beads conjugated to anti-mouse IgG antibody. These beads are used to capture mouse IgG antibodies (targeting the Fc region).
Price Range : 612.00 USD - 25100.00 USD
AlphaLisa Analyte 2

CCL3 / MIP-1α (human), lyophilized (AlphaLISA)

Human C-C Motif Chemokine 3 / Macrophage Inflammatory Protein-1alpha (CCL3 / MIP-1α) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human CCL3/MIP-1α kit, but can be ordered separately.
List Price : 257.00 USD
SureFire principle

AlphaScreen SureFire Phospho-p38 MAPK Kit

The AlphaScreen® SureFire® p-38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1703.00 USD - 18200.00 USD
AlphaLISA immunoassay kits

Heme oxygenase 1 AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human heme oxygenase 1 (HO-1) enables the quantitative determination of human HO-1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 451.00 USD - 9900.00 USD

Anti-mouse IgG (Fc specific) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-mouse IgG antibody. These beads can be used to capture mouse IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.
Price Range : 613.00 USD - 25200.00 USD
AlphaLisa Analyte 2

Mesothelin (human), lyophilized (AlphaLISA)

Human mesothelin standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human Mesothelin Detection Kit, but can also be ordered separately.
List Price : 421.00 USD

ApoE (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoE enables the quantitative determination of Apolipopriein E (ApoE) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimum cross-reactivity with ApoA1, ApoB, and ApoCIII. Crossreactivity with other species has not tested.
Price Range : 644.00 USD - 19300.00 USD
AlphaLISA immunoassay kits

NT-proBNP (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human NT-proBNP enables the quantitative determination of human N-terminal prohormone of brain natriuretic peptide (NT-proBNP) in buffer, serum, or plasma using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 782.00 USD - 11100.00 USD
AlphaLisa Analyte 2

ApoB (human), lyophilized (AlphaLISA)

Human ApoB standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human ApoB Detection Kit, but can also be ordered separately.
List Price : 308.00 USD
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IL2 Detection Kit

The AlphaPlex™ 645 (Samarium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
Price Range : 633.00 USD - 10500.00 USD

IgA (mouse) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of mouse IgA in cell culture/non-mouse serum allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 646.00 USD - 10100.00 USD
AlphaPlex Samarium Acceptor Beads

Anti-FLAG AlphaPlex-645 Acceptor Beads

AlphaPlex-645 (Samarium) Acceptor beads conjugated to anti-FLAG antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 612.00 USD - 25100.00 USD
AlphaLISA immunoassay kits

Ghrelin (active) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for active human ghrelin enables the quantitative determination of active ghrelin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 625.00 USD - 18800.00 USD
AlphaLISA immunoassay kits

C-Reactive Protein (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-Reactive Protein (CRP) Detection Kit is designed for detection and quantitation of human CRP in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1585.00 USD - 10500.00 USD
SureFire principle

AlphaScreen SureFire ATF1 (p-Ser63) Assay Kit

The AlphaScreen® SureFire® p-ATF1 (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-ATF1 (phosphorylated on Ser63) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr37/Thr46) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1703.00 USD - 18200.00 USD
AlphaLisa Analyte 2

CCL18 (human), lyophilized (AlphaLISA)

Recombinant human CCL18 standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human CCL18 detection kit, but can also be ordered separately.
List Price : 759.00 USD

IL-21 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-21 enables the quantitative determination of human interleukin-12 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 625.00 USD - 15000.00 USD

Anti-rat IgG (Fc specific) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-rat IgG antibody. These beads can be used to capture rat IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.
Price Range : 613.00 USD - 25200.00 USD
AlphaLISA immunoassay kits

Mesothelin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human mesothelin enables the quantitative determination of human mesothelin (CAK1, ERC) in serum, buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 782.00 USD - 15000.00 USD
AlphaLISA immunoassay kits

Cardiac Troponin I (human) AlphaLISA Detection Kit

The AlphaLISA® Human cardiac Troponin I Detection Kit is designed for detection and quantitation of human cardiac Troponin I in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 3036.00 USD - 23700.00 USD

AlphaScreen FITC (Fluorescein) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-FITC conjugated AlphaScreen Acceptor beads. 
Price Range : 200.00 USD - 14400.00 USD
AlphaLISA immunoassay kits

PEG IgM AlphaLISA Detection Kit

The AlphaLISA anti-PEG IgM Detection Kit is designed for the quantitative determination of anti-PEG IgM antibodies in sera using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 792.00 USD - 17800.00 USD
AlphaLISA immunoassay kits

Transferrin (human) AlphaLISA Detection Kit

The AlphaLISA® Human Transferrin Detection Kit is designed for detection and quantitation of human transferrin in cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 646.00 USD - 10100.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Thr1343) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II-α (p-Thr1343) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II-α (phosphorylated on Thr1343) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD
AlphaLISA immunoassay kits

ApoA1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoA1 is designed for the quantitative determination of ApoA1 in human serum, human plasma and cell supernantants using a homogeneous AlphaLISA assay (no wash steps). Neglible cross reactivity was observed with ApoCIII, ApoE, or ApoB.
Price Range : 644.00 USD - 19300.00 USD
AlphaLisa Analyte 2

IL-17A (mouse), lyophilized (AlphaLISA)

Mouse Interleukin-17 (IL-17A) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse/Rat IL-17A kit, but can be ordered separately.
List Price : 257.00 USD

Anti-bovine IgG AlphaLISA Acceptor Beads

AlphaLISA Acceptor beads conjugated to anti-bovine IgG (total) antibody.
Price Range : 864.00 USD - 29200.00 USD
Alpha donor antibody coated

Anti-Human IgG Alpha Donor beads

Alpha Donor beads conjugated to mouse anti-human IgG (Fc-specific) antibody. This bead can be used to capture human IgG antibodies containing an Fc-region for Alpha no-wash protein detection, Fc-receptor binding, antibody detection, antibody binding, and other applications.
Price Range : 509.00 USD - 9200.00 USD
PerkinElmer

AlphaLISA TruHits Kit

Control beads for identifying false positives and interfering compounds in AlphaLISA® no-wash assays.
Price Range : 596.00 USD - 4138.00 USD

Myoglobin (human) AlphaLISA Detection Kit

The AlphaLISA® Human Myoglobin Detection Kit is designed for detection and quantitation of human myoglobin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 3036.00 USD - 23700.00 USD
AlphaLISA assay principle

Osteoprotegerin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for total osteoprotegerin enables the quantitative determination of human osteoprotegerin in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 644.00 USD - 19300.00 USD

EGFR (human) AlphaLISA Detection Kit

The AlphaLISA® EGFR Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor Receptor (EGFR) in cell culture media, human serum, and other sample types using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 724.00 USD - 10500.00 USD
AlphaLISA immunoassay kits

IL-10 (bovine/ovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-10 enables the detection and quantitation of bovine IL-10 (bIL10) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). This kit also detects Ovine IL-10.
Price Range : 725.00 USD - 13100.00 USD
SureFire principle

AlphaScreen SureFire cJun (p-Ser63) Assay Kit

The AlphaScreen® SureFire® p-c-Jun (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-c-Jun (phosphorylated on Ser63) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr70) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr70) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr70) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD
AlphaLISA immunoassay kits

CXCL1/GROα (human) AlphaLISA Detection Kit

The AlphaLISA® Human CXCL1/GROα Detection Kit is designed for detection and quantitation of Human CXCL1/GROα in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1584.00 USD - 10500.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

The AlphaLISA® SureFire® Ultra p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 29200.00 USD
AlphaLISA immunoassay kits

CCL18 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CCL18 enables the quantitative determination of human chemokine (C-C motif) ligand 18 in buffer, human serum, cell media, and PBMC culture supernatant samples using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 782.00 USD - 11100.00 USD

IL-7 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin 7 (IL7) Detection Kit is designed for detection and quantitation of human IL-7 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1585.00 USD - 10500.00 USD
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr389) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1703.00 USD - 18200.00 USD

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