PerkinElmer

Alpha Reagents

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Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 974 Products and Services

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BDNF (human) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for detection and quantitation of Brain Derived Neurotrophic Factor (BDNF) in serum, cell culture supernatants, and other samples.
Price Range : 695.00 USD - 10100.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SYK (Tyr525/526) Assay Kit

The AlphaLISA® SureFire® Ultra p-SYK (Tyr525/526) assay is a sandwich immunoassay for quantitative detection of phospho-SYK (phosphorylated on Tyr525/526) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD

BMP-9 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human BMP-9 enables the quantitative determination of human Bone Morphogenetic Protein 9 (BMP-9) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 600.00 USD - 18000.00 USD
PerkinElmer

AlphaLISA SureFire Ultra 4EBP1 pT37/46 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-4E-BP1 (Thr37/46) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-4E-BP1 assay kit, but can also be ordered separately.
List Price : 21.00 USD
AlphaLISA SureFire Ultra kits

Phospho-ErbB2 (Tyr1221/1222) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ p-ErbB2 (Tyr1221/1222) assay is a sandwich immunoassay for quantitative detection of phospho-ErbB2 (phosphorylated on Tyr1221/1222) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 29500.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT4 (Tyr693) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT4 (Tyr693) assay is a sandwich immunoassay for quantitative detection of endogenous STAT4 phosphorylated at Tyr693 in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
AlphaLisa Analyte 2

CXCL13 (human), lyophilized (AlphaLISA)

Recombinant human CXCL13 standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human CXCL13 detection kit, but can also be ordered separately.
List Price : 700.00 USD
AlphaLISA immunoassay kits

Insulin (human) AlphaLISA Detection Kit

The AlphaLISA® Human Insulin Detection Kit is designed for detection and quantitation of human insulin in cell culture supernatants, serum, and plasma using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 695.00 USD - 9700.00 USD
AlphaLisa Analyte 2

Insulin (human), lyophilized (AlphaLISA)

Human insulin, lyophilized, 0.01 IU. Human insulin is included in the AlphaLISA® human insulin kits, but can also be ordered separately as a standalone item. One vial contains an amount of human insulin sufficient for performing 10 standard curves.
List Price : 247.00 USD
AlphaPlex-545-antibody-bead2

Anti-FLAG AlphaPlex-545 (Tb) Acceptor beads

AlphaPlex-545 (Terbium) Acceptor beads conjugated to anti-FLAG antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 587.00 USD - 24100.00 USD
AlphaLisa Analyte 2

IL-8 (bovine), lyophilized (AlphaLISA)

Bovine IL-8 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Bovine IL-8 Detection Kit, but can be ordered separately.
List Price : 247.00 USD
AlphaLISA immunoassay kits

Somatostatin (human) AlphaLISA Detection Kit

The AlphaLISA® Somatostatin Detection Kit is designed for detection and quantitation of somatostatin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 695.00 USD - 10000.00 USD

D-dimer (human) AlphaLISA Detection Kit

The AlphaLISA® Human D-dimer Detection Kit is designed for detection and quantitation of human D-dimer in citrate plasma, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Please note the kit shows some cross-reactivity to D-monomer.
Price Range : 2912.00 USD - 22800.00 USD
AlphaLisa Analyte 2

Vimentin (human), liquid (AlphaLISA)

Human vimentin standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human Vimentin Detection Kit, but can also be ordered separately.
List Price : 404.00 USD
AlphaLISA immunoassay kits

TNFα (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine TNFα enables the detection and quantitation of porcine TNFα in porcine serum, plasma, and, cell lysyate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimal cross-reactivity with human and bovine TNFα and no cross reactivity towards porcine IFNγ.
Price Range : 622.00 USD - 20200.00 USD

Anti-HA AlphaLISA Acceptor Beads

AlphaLISA Acceptor beads conjugated to anti-HA (hemagglutinin) antibody. These beads can be used to capture HA-tagged proteins and peptides.
Price Range : 588.00 USD - 24100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-Ribosomal Protein S6 (Ser240/244) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-Ribosomal Protein S6 (Ser240/244) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaLISA immunoassay kits

TNFα (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human TNFα Biotin-Free Detection Kit is designed for the quantitative detection of human TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 695.00 USD - 10100.00 USD

AlphaScreen General IgG (Protein A) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Protein A conjugated AlphaScreen Acceptor beads.
Price Range : 90.00 USD - 13800.00 USD

IL-2 (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Interleukin-2 (IL-2) Detection Kit is designed for detection and quantitation of mouse IL-2 in serum, buffered solution, or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1520.00 USD - 10100.00 USD

IgA (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgA in serum, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 645.00 USD - 10300.00 USD
AlphaLISA Cellular Epigenetics kits

AlphaLISA di/mono-methylated-Histone H3 Lysine 27 (H3K27me2-1) Cellular Detection Kit

This kit is designed for the detection of di/mono-methylated Histone H3 Lysine 27 (H3K27me2-1) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 695.00 USD - 12400.00 USD
SureFire principle

AlphaScreen SureFire S6RP (p-Ser235;Ser236) Assay Kit

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser235/236) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser235/236) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately, in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLISA SureFire Ultra kits

Phospho-JNK1/2/3 (Thr183/Tyr185) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.
Price Range : 489.00 USD - 28400.00 USD

Anti-bovine IgG2 AlphaLISA Acceptor Beads

AlphaLISA Acceptor beads conjugated to anti-bovine IgG2 antibody. These beads can be used to capture bovine IgG2 antibodies.
Price Range : 828.00 USD - 28000.00 USD
AlphaLisa Analyte 2

B7-H5/VISTA (human), lyophilized (AlphaLISA)

Human VISTA standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human VISTA Detection Kit, but can also be ordered separately.
List Price : 404.00 USD

IL-13 (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for IL13 is designed for the quantitative determination of IL13 in porcine serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimal cross-reactivity with human IL13.
Price Range : 696.00 USD - 10300.00 USD
AlphaLisa Analyte 2

Mesothelin (human), lyophilized (AlphaLISA)

Human mesothelin standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human Mesothelin Detection Kit, but can also be ordered separately.
List Price : 404.00 USD
AlphaLISA SureFire Ultra kits

Phospho-p38 MAPK (Thr180/Tyr182) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Tyr180/182) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 29500.00 USD
SureFire principle

AlphaScreen SureFire IKKb (p-Ser177/Ser181) Assay Kit

The AlphaScreen® SureFire® p-IKKα (Ser177/181) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser177/181) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD

Protein L AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to Protein L. These beads can be used to capture antibodies that bind Protein L for AlphaLISA no-wash assays.
Price Range : 588.00 USD - 24100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-NF-κB p65 (Ser536) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-NF-κB p65 (Ser536) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD

AlphaLISA Anti-O-linked GlcNAc Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against O-linked n-acetylglucosamine (O-linked GlcNAc). These beads can be used for AlphaLISA no-wash assays.
Price Range : 1420.00 USD - 25900.00 USD

AlphaScreen Omnibeads

Single bead instrument control that contains the chemistry of both Alpha Donor beads and AlphaScreen Acceptor beads.
AlphaLisa Analyte 2

Human IgG, lyophilized (AlphaLISA)

Human IgG, lyophilized, 3 µg. Human IgG is included in the AlphaLISA® human IgG kits, but can also be ordered separately as a standalone item. One vial contains an amount of human IgG sufficient for performing 10 standard curves.
List Price : 247.00 USD
AlphaLisa Analyte 2

Cry2A analyte, lyophilized (AlphaLISA)

Cry2A standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Cry2A kit, but can be ordered separately.
List Price : 330.00 USD
PerkinElmer

AlphaLISA SureFire Ultra VEGFR2 pY1175 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-VEGFR2 (Tyr1175) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-VEGFR2 assay kit, but can also be ordered separately.
List Price : 21.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total GAPDH (Human/Mouse) Assay Kit

The AlphaLISA® SureFire® Ultra Mouse/Human GAPDH assay is a sandwich immunoassay for quantitative detection of human or mouse GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay.
Price Range : 489.00 USD - 28400.00 USD

Anti-mouse IgG (Fc specific) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-mouse IgG antibody. These beads can be used to capture mouse IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.
Price Range : 588.00 USD - 24100.00 USD
alphascreen surefire total

AlphaScreen SureFire Total Topo Iia Assay Kit

The AlphaScreen® SureFire® total Topoisomerase II-α assay is a sandwich immunoassay for quantitative detection of Topoisomerase II-α (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaPlex Samarium Acceptor Beads

Anti-FLAG AlphaPlex-645 Acceptor Beads

AlphaPlex-645 (Samarium) Acceptor beads conjugated to anti-FLAG antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 587.00 USD - 24100.00 USD

AlphaPlex-645 TruHits (Sm) Kit

AlphaPlex™ 645 (Samarium) TruHits kit for use as a control for interference in Alpha no-wash multiplexing assays. This kit provides enough reagents to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume.
Price Range : 572.00 USD - 3969.00 USD
alphascreen surefire total

AlphaScreen SureFire Total NFkB p65 Assay Kit

The AlphaScreen® SureFire® total NFκB assay is a sandwich immunoassay for quantitative detection of NFκB (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLisa Analyte 2

TSLP (human), lyophilized (AlphaLISA)

Human TSLP standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human TSLP Detection Kit, but can also be ordered separately.
List Price : 295.00 USD

Galectin-9 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human galectin-9 enables the quantitative determination of human galectin-9 (Gal-9) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 750.00 USD - 14400.00 USD
AlphaLISA immunoassay kits

N-cadherin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human N-cadherin enables the detection and quantitation of human N-Cadherin in tissue homogenates and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 459.00 USD - 10000.00 USD
AlphaLISA SureFire Ultra kits

Phospho-mTOR (Ser2448) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha no-wash technology.
Price Range : 489.00 USD - 28400.00 USD

BTLA (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human BTLA enables the quantitative determination of human B- and T-lymphocyte attenuator (BTLA, CD272) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 750.00 USD - 14400.00 USD
AlphaLISA Buffer

AlphaLISA HiBlock Buffer

Pre-formulated buffer that can be used as a high blocking buffer for Alpha analyte detection assays. This buffer is included in select AlphaLISA immunoassay kits, but can also be purchased to develop your own Alpha assay.
Price Range : 202.00 USD - 384.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 Human IL17 Detection Kit

The AlphaPlex™ 545 (Terbium) Human IL17 Detection Kit is designed for the quantitative determination of human IL17 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 359.00 USD - 10000.00 USD

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