PerkinElmer

Alpha Reagents

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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AlphaLISA immunoassay kits

TDP-43 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for TDP-43 is designed for the quantitative determination of Transactive response DNA binding protein 43 (TDP43) in human serum, CSF, and, cell lysate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 622.00 USD - 20200.00 USD
AlphaLISA SureFire Ultra kits

Phospho-eIF2α (Ser51) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra p-IGF Receptor β (Tyr1135/1136) assay is a sandwich immunoassay for quantitative detection of phospho-IGF Receptor β (phosphorylated on Tyr1135/1136) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 29500.00 USD
AlphaLISA immunoassay kits

Chloramphenicol AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for Chloramphenicol enables the quantitative determination of Chloramphenicol in buffered solution, sample extracts, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 428.00 USD - 10100.00 USD
PerkinElmer

AlphaLISA SureFire Ultra p-eIF2α (Ser51) Lysate

Extra positive and negative control lysates for the AlphaLISA® SureFire® Ultra phospho-eIF2α (Ser51) assay.
List Price : 21.00 USD
AlphaLISA immunoassay kits

BCL-2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for BCL-2 enables the detection and quantitation of human BCL-2 in serum, buffered solution, or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). This kit is not cross reactive with mouse Bcl-2.
Price Range : 622.00 USD - 20200.00 USD
AlphaPlex Samarium Acceptor Beads

Unconjugated AlphaPlex™ 545 Acceptor Beads

Unconjugated AlphaPlex 645 (Samarium) Acceptor beads bear reactive aldehyde groups at their surface, allowing you to conjugate a wide variety of reagents using a simple reductive amination protocol.
Price Range : 1343.00 USD - 28300.00 USD
AlphaPlex Samarium Acceptor Beads

Protein A AlphaPlex™ 645 (Sm) Acceptor Beads

AlphaPlex-645 (Samarium) Acceptor beads conjugated to Protein A. These beads are used to capture immunoglobulins through their heavy chain (Fc region) allowing optimal orientation and antigen detection. Protein A has higher affinity for rabbit, pig, dog and cat IgG.
Price Range : 587.00 USD - 24100.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

The AlphaLISA® SureFire® Ultra p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD

AlphaScreen FITC (Fluorescein) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-FITC conjugated AlphaScreen Acceptor beads. 
Price Range : 192.00 USD - 13800.00 USD
SureFire principle

AlphaScreen SureFire CREB (p-Ser133) Assay Kit

The AlphaScreen® SureFire® p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD
AlphaLisa Analyte 2

p53 (human), lyophilized (AlphaLISA)

p53 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human p53 Detection Kit, but can be ordered separately.
List Price : 300.00 USD
AlphaLISA immunoassay kits

Adiponectin (human) AlphaLISA Detection Kit

The AlphaLISA® Human Adiponectin Detection Kit is designed for detection and quantitation of human adiponectin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1520.00 USD - 10100.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total GAPDH (Human/Mouse) Assay Kit

The AlphaLISA® SureFire® Ultra Mouse/Human GAPDH assay is a sandwich immunoassay for quantitative detection of human or mouse GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay.
Price Range : 489.00 USD - 28400.00 USD

HLA-C (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human HLA-C enables the quantitative determination of human MHC class I antigen C (HLA-C) in buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 750.00 USD - 14400.00 USD
AlphaLISA SureFire Ultra kits

Phospho-SLP-76 (Ser376) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.
Price Range : 489.00 USD - 28400.00 USD

AlphaScreen Mouse IgG Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-mouse IgG conjugated AlphaScreen Acceptor beads.
Price Range : 180.00 USD - 13800.00 USD
AlphaLISA immunoassay kits

C-Reactive Protein (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-Reactive Protein (CRP) Detection Kit is designed for detection and quantitation of human CRP in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1520.00 USD - 10100.00 USD
SureFire principle

AlphaScreen SureFire Phospho-MEK 1 Kit

The AlphaScreen® SureFire® p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD
AlphaLisa Analyte 2

Aflatoxin, in DMSO (AlphaLISA)

Aflatoxin standard for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Aflatoxin Detection Kit, but can be ordered separately.
List Price : 240.00 USD
SureFire principle

AlphaScreen SureFire CHK1 (p-Ser345) Assay Kit

The AlphaScreen® SureFire® p-CHK1 (Ser345) assay is a sandwich immunoassay for quantitative detection of phospho-CHK1 (phosphorylated on Ser345 in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
PerkinElmer

AlphaLISA SureFire Ultra p-IGF Receptor β (Tyr1135/1136) Lysate

Extra positive and negative control lysates for the AlphaLISA® SureFire® Ultra phospho-IGF Receptor β (Tyr1135/1136) assay.
List Price : 21.00 USD
AlphaLisa Analyte 2

TSLP (mouse), lyophilized (AlphaLISA)

Mouse Thymic stromal lymphopoietin (TSLP) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA mouse TSLP Detection Kit, but can also be ordered separately.
List Price : 295.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT6 (Tyr641) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT6 (Tyr641) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IL17 Detection Kit

The AlphaPlex™ 645 (Samarium) Human IL17 Detection Kit is designed for the quantitative determination of human IL17 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
Price Range : 359.00 USD - 10000.00 USD
AlphaLISA immunoassay kits

LDHA (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human lactate dehydrogenase subunit A (LDHA) enables the quantitative determination of human LDHA in cell lysates and serum using a homogeneous AlphaLISA assay (no wash steps). Please note this is NOT a cell cytotoxicity assay, as the assay does not measure secreted LDH.
Price Range : 728.00 USD - 14000.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-AKT (Ser473) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-AKT (Ser473) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaLISA immunoassay kits

IL-8 (bovine/ovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-8 enables the detection and quantitation of bovine IL8 (bIL8) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). This kit is 100% cross-reactive with Ovine IL-8.
Price Range : 695.00 USD - 10000.00 USD
AlphaLisa Analyte 2

IL-22 (human), lyophilized (AlphaLISA)

Human IL-22 standard, 1 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA human/rat IL-22 detection kits, but can also be ordered separately.
List Price : 247.00 USD
PerkinElmer

AlphaLISA SureFire Ultra p-p38 MAPK (Thr180/Tyr182) Lysate

Extra positive and negative control lysates for the AlphaLISA® SureFire® Ultra phospho-p38 MAPK (Thr180/Tyr182) assay.
List Price : 21.00 USD

AlphaScreen Rabbit IgG Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-rabbit IgG conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
Price Range : 180.00 USD - 13800.00 USD
AlphaLisa Analyte 2

C-peptide (human), lyophilized (AlphaLISA)

Recombinant human C-peptide, lyophilized, for use in AlphaLISA® no-wash immunoassay standard curves. This standard is already provided in ALphaLISA C-peptide immunoassay kits, but can be ordered separately.
List Price : 240.00 USD
SureFire principle

AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser112) Kit

The AlphaScreen® SureFire® p-BAD (Ser112) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser112) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD

Trefoil factor 3 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Trefoil Factor 3 (TFF3) Detection Kit is designed for detection and quantitation of human TFF3 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1520.00 USD - 10100.00 USD

p53 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human p53 enables the detection and quantitation of human p53 in serum, buffered solution, or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
List Price : 19600.00 USD
AlphaLisa Analyte 2

G-CSF (human), lyophilized (AlphaLISA)

Human Granulocyte Colony-Stimulating Factor (G-CSF) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human G-CSF kit, but can be ordered separately.
List Price : 247.00 USD
AlphaLISA binding assay principle

PD-1/PD-L1 (human) AlphaLISA Binding Kit

The AlphaLISA® PD-1/PD-L1 Binding Kit is designed for the detection of binding activity between Human PD-1 and PD-L1, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of therapeutics which competitively inhibit PD-1/PD-L1 interaction.
Price Range : 695.00 USD - 9700.00 USD

AlphaScreen® P-Tyr-100 (Phosphotyrosine) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine P-Tyr-100 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 180.00 USD - 13800.00 USD
Alphaplex-545-terbium-glutathione

Glutathione AlphaPlex™ 545 Acceptor Beads

AlphaPlex™-545 (Terbium) Acceptor beads conjugated to glutathione, for use in Alpha no-wash multiplexing assays.
Price Range : 587.00 USD - 24100.00 USD
SureFire principle

AlphaScreen SureFire Akt 1 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLISA immunoassay kits

IL-13 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse IL-13 enables the quantitative determination of mouse interleukin-13 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 600.00 USD - 14400.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT3 (Tyr705) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT3 (Tyr705) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD

Anti-rat IgG (Fc specific) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-rat IgG antibody. These beads can be used to capture rat IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.
Price Range : 588.00 USD - 24100.00 USD
AlphaLISA SureFire Ultra kits

Phospho-GSK-3β (Ser9) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-GSK-3β (Ser9) assay is a sandwich immunoassay for quantitative detection of endogenous GSK-3β phosphorylated at Ser9 in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
PerkinElmer

AlphaLISA SureFire Ultra Total Cofilin Lysate

Extra control lysates for the AlphaLISA® SureFire® Ultra total cofilin assay.
List Price : 21.00 USD

Anti-acetyl-Histone H3 Lysine 9 (H3K9ac) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 acetylated at lysine 9 (H3K9Ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 1478.00 USD - 26900.00 USD
AlphaLisa Analyte 2

CXCL12/SDF-1 (human), liquid (AlphaLISA)

Human CXCL12 (SDF-1) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human CXCL12 Detection Kit, but can also be ordered separately.
List Price : 233.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-p70 S6K (Thr389) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-p70 S6K (Thr389) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaLISA immunoassay kits

FCGR3A/CD16a (176Val/V158) AlphaLISA Binding Kit

This kit is designed for the detection of binding activity between human FCGR3A (176Val) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
Price Range : 695.00 USD - 10600.00 USD

AlphaScreen Phosphotyrosine (PT66) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PT66 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 227.00 USD - 13800.00 USD

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