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Alpha

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Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you:

  • AlphaLISA – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 937 Products and Services

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AlphaLISA immunoassay kits

IL-1β (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Interleukin-1ß (IL-1ß) Detection Kit is designed for detection and quantitation of mouse IL-1ß in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
AlphaLISA assay principle

TSH (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human TSH enables the quantitative determination of human thyroid stimulating hormone (TSH) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 600.00 USD - 18000.00 USD
AlphaPlex Samarium Acceptor Beads

Anti-6X His AlphaPlex 645 (Sm) Acceptor beads

AlphaPlex™-645 (Samarium) Acceptor beads conjugated to anti-6XHis antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 570.00 USD - 23400.00 USD
AlphaLISA immunoassay kits

TNFα (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human TNFα Biotin-Free Detection Kit is designed for the quantitative detection of human TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 675.00 USD - 9800.00 USD
AlphaLisa Analyte 2

IL-4 (mouse), lyophilized (AlphaLISA)

Mouse IL-4 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA mouse IL-4 Detection Kit, but can also be ordered separately.
List Price : 287.00 USD

AlphaScreen C-MYC Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-c-myc-conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
Price Range : 175.00 USD - 13400.00 USD
AlphaLISA immunoassay kits

MMP-3 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Matrix Metalloproteinase-3 (MMP3) Detection Kit is designed for detection and quantitation of human MMP3 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP3 and pro-MMP3.
Price Range : 2827.00 USD - 22100.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT4 (Tyr693) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT4 (Tyr693) assay is a sandwich immunoassay for quantitative detection of endogenous STAT4 phosphorylated at Tyr693 in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27600.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total ERK 1/2 Assay Kit

The AlphaLISA® SureFire® Ultra™ Total ERK 1/2 assay is a sandwich immunoassay for quantitative detection of ERK 1/2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 475.00 USD - 27500.00 USD
AlphaScreen Acceptor Beads

AlphaScreen Unconjugated Acceptor Beads

Unconjugated AlphaScreen® Acceptor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own AlphaScreen Acceptor beads for use in Alpha no-wash assays.
Price Range : 349.00 USD - 9800.00 USD
AlphaLisa Analyte 2

Albumin (human), lyophilized (AlphaLISA)

Recombinant human albumin, lyophilized, for use in AlphaLISA no-wash immunoassay standard curves. This standard is already provided in ALphaLISA albumin immunoassay kits, but can be ordered separately.
List Price : 514.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Caspase 9 (Ser196) Kit

The AlphaScreen® SureFire® p-Caspase 9 (Ser196) assay is a sandwich immunoassay for quantitative detection of phospho-Caspase 9 (phosphorylated on Ser196) in cellular lysates using Alpha Technology
Price Range : 1449.00 USD - 15700.00 USD
AlphaPlex Samarium Acceptor Beads

Anti-FLAG AlphaPlex-645 Acceptor Beads

AlphaPlex-645 (Samarium) Acceptor beads conjugated to anti-FLAG antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 570.00 USD - 23400.00 USD
AlphaLISA immunoassay kits

MMP-7 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human MMP7 enables the detection and quantitation of human pro- and active MMP-7 (hMMP-7) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 433.00 USD - 9500.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 Human IL1β Detection Kit

The AlphaPlex™ 545 Human IL1β Detection Kit is designed for the quantitative determination of human IL1β together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
Price Range : 571.00 USD - 9700.00 USD
PerkinElmer

AlphaLISA SureFire Insulin recepeptor pY1150/1 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-Insulin Receptor β (Tyr1150/1151) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-Insulin Receptor β assay kit, but can also be ordered separately.
List Price : 21.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-MEK1 (Ser218/222) Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha no-wash technology.
List Price : 27600.00 USD
AlphaLISA immunoassay kits

PIGF (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human placental growth factor (PIGF) enables the quantitative determination of human PIGF (PGF) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD
AlphaLISA immunoassay kits

CTLA-4 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) enables the quantitative determination of human CTLA-4 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 582.00 USD - 14000.00 USD
AlphaLISA Cellular Epigenetics kits

AlphaLISA Di-Methyl-Histone H3 Lysine 4 (H3K4me2) Cellular Detection Kit

This kit is designed for the detection of di-methylated Histone H3 Lysine 4 (H3K4me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 675.00 USD - 12000.00 USD
AlphaLISA immunoassay kits

IL-18 (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-18 enables the quantitative determination of bovine interleukin 18 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IL2 Detection Kit

The AlphaPlex™ 645 (Samarium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
Price Range : 589.00 USD - 9700.00 USD
SureFire principle

AlphaScreen SureFire MKK4 (p-Ser257;Thr261) Assay Kit

The AlphaScreen® SureFire® p-MKK4 (Ser257/Thr261) assay is a sandwich immunoassay for quantitative detection of phospho-MKK4 (phosphorylated on Ser257/Thr261) in cellular lysates using Alpha Technology.
Price Range : 1448.00 USD - 15600.00 USD
AlphaLISA immunoassay kits

IL-13 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse IL-13 enables the quantitative determination of mouse interleukin-13 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 582.00 USD - 14000.00 USD
AlphaLISA immunoassay kits

ApoA1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoA1 is designed for the quantitative determination of ApoA1 in human serum, human plasma and cell supernantants using a homogeneous AlphaLISA assay (no wash steps). Neglible cross reactivity was observed with ApoCIII, ApoE, or ApoB.
Price Range : 600.00 USD - 18000.00 USD
AlphaLISA immunoassay kits

IL-33 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-33 enables the quantitative determination of human interleukin-33 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 582.00 USD - 14000.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total GAPDH Assay Kit

The AlphaLISA® SureFire® Ultra™ Total GAPDH assay is a sandwich immunoassay for quantitative detection of GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for cell number.
Price Range : 475.00 USD - 27500.00 USD
AlphaLisa Analyte 2

IL-13 (porcine), lyophilized (AlphaLISA)

Porcine IL-13 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA IL-13 Detection Kit, but can be ordered separately.
List Price : 233.00 USD
AlphaLISA immunoassay kits

Alpha-fetoprotein (human) AlphaLISA Detection Kit

The AlphaLISA® Human Alpha-Fetoprotein (AFP) Detection Kit is designed for detection and quantitationof human AFP in cell culture medium and serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
AlphaLisa Analyte 2

Amyloid β 1-40 (human), lyophilized (AlphaLISA)

Human Amyloid β 1-40, lyophilized, 0.3 µg. Amyloid β 1-40 is included in the AlphaLISA® human Amyloid β 1-40 kits, but can also be ordered separately as a standalone item. One vial contains an amount of human Amyloid β 1-40 sufficient for performing 10 standard curves.
List Price : 240.00 USD
AlphaLISA immunoassay kits

D-dimer (human) AlphaLISA Detection Kit

The AlphaLISA® Human D-dimer Detection Kit is designed for detection and quantitation of human D-dimer in citrate plasma, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Please note the kit shows some cross-reactivity to D-monomer.
Price Range : 2827.00 USD - 22100.00 USD
AlphaLISA immunoassay kits

Ghrelin (active) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for active human ghrelin enables the quantitative determination of active ghrelin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 582.00 USD - 17500.00 USD
AlphaLisa Analyte 2

p53 (human), lyophilized (AlphaLISA)

p53 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human p53 Detection Kit, but can be ordered separately.
List Price : 300.00 USD
SureFire principle

AlphaScreen SureFire STAT1 (p-Tyr701) Assay Kit

The AlphaScreen® SureFire® p-STAT1 (p-Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD

Anti-methyl-Histone H3 Lysine 4 (H3K4me1-2) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 mono- or di-methylated at lysine 4 (H3K4me1-2). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 1379.00 USD - 25100.00 USD

AlphaLISA Anti-unmodified Histone H3 Lysine 4 (H3K4) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 unmodified against lysine 4 (H3K4). These beads can be used for no-wash AlphaLISA epigenetic eraser assays.
Price Range : 1379.00 USD - 25100.00 USD
AlphaLISA immunoassay kits

ANGPTL4 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ANGPTL4 enables the quantitative determination of ANGPTL4 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD
AlphaLISA immunoassay kits

Fibronectin (human plasma) AlphaLISA Detection Kit

The AlphaLISA® Human Plasma Fibronectin Detection Kit is designed for detection and quantitation of human plasma fibronectin (hFN) in human serum, plasma, and, cell lysate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 433.00 USD - 9500.00 USD
Alpha SureFire Ultra Multiplex kits

Alpha SureFire Ultra Multiplex p-AKT (Ser473) (directly coupled) Kit

Single target-only kit for multiplexing to other AlphaLISA SureFire Ultra assay kits. The Alpha SureFire® Ultra Multiplex kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of another protein, phospho or total. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm).
Price Range : 475.00 USD - 27600.00 USD

Protein G AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to Protein G. These beads can be used to capture antibodies that associate with Protein G.
Price Range : 571.00 USD - 23400.00 USD
AlphaLISA immunoassay kits

MMP13 (human) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for detection and quantitation of human MMP13 (matrix metalloproteinase-13, or Collagenase 3) in serum, plasma, cell culture supernatants, and other samples.
Price Range : 626.00 USD - 9700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-4E-BP1 (Thr37/46) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-4E-BP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD
AlphaLISA immunoassay kits

IL-15 (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA® Human IL-15 Biotin-Free Detection Kit is designed for the quantitative detection of human Interleukin 15 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
Price Range : 433.00 USD - 9500.00 USD

Protein L AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to Protein L. These beads can be used to capture antibodies that bind Protein L for AlphaLISA no-wash assays.
Price Range : 571.00 USD - 23400.00 USD

Anti-H3K36me2 AlphaLISA Acceptor Beads

AlphaLISA Acceptor beads conjugated to anti-H3K36me2 antibody. These beads can be used for AlphaLISA epigenetic assays using histone or nucleosome substrates.
Price Range : 1379.00 USD - 25100.00 USD
AlphaLISA immunoassay kits

PCSK9 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Proprotein Convertase Subtilisin / Kexin Type 9 (PCSK9) Detection Kit is designed for detection and quantitation of human PCSK9 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. One antibody targets the pro-domain of PCSK9.
Price Range : 1476.00 USD - 9800.00 USD
AlphaLISA immunoassay kits

VCAM-1 (human) AlphaLISA Detection Kit

The AlphaLISA® VCAM-1 Detection Kit is designed for detection and quantitation of Intracellular Adhesion Molecule 1 (VCAM-1) in cell culture media, serum, and plasma using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 626.00 USD - 9800.00 USD

AlphaScreen FLAG (M2) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-FLAG conjugated AlphaScreen Acceptor beads. 
Price Range : 175.00 USD - 13400.00 USD

AlphaScreen GST Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-GST conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
Price Range : 175.00 USD - 13400.00 USD
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human Insulin Detection Kit

The AlphaPlex™ 645 (Samarium) Human Insulin Detection Kit is designed for the quantitative determination of human insulin together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 447.00 USD - 9700.00 USD

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